Sürdürülebilir Kalkınma Amaçları
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- MakaleYalnız künye2023Investigation of the effect of liquid phase of digested biomass on Phaseolus vulgaris L. var. ellipticus Mart under NaCl stress
Çiftçi, Fida; Acet, Tuba; Saribiyik, Oǧuz Yunus
The factors such as global warming, wild irrigation, and synthetic fertilisers pollute the soil and increase salinity. The salinity is defined as salt stress for plants, and plants have developed various tolerance mechanisms to survive under salt stress conditions. Therefore, efforts to increase the tolerance of salt sensitive plants such as beans (Phaseolus vulgaris) are considered important. Additionally, considering problems that appear depending on global warming, understanding the tolerance mechanisms, and activating them by using natural and sustainable solutions are important for plant growth and preventing lower agricultural productivity. In this study, the effect of biofertiliser obtained from a small-scale bioreactor on a plant exposed to salt stress on the development of morphological, physiological, and biochemical properties of the model plant, Phaseolus vulgaris L. var. ellipticus Mart (Pve) were investigated. The biofertiliser application increased the amount of chlorophyll, flavonoids, anthocyanin, and proline while the amount of hydrogen peroxide and lipid peroxidation decreased in the plant. This change enhanced the enzyme activities of catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX) and the plant formed tolerance to the salt stress. As a result, the biofertiliser application to the plant may activate the tolerance mechanism against salt stress.
- MakaleAçık Erişim2020SOS5 gene-abscisic acid crosstalk and their interaction with antioxidant system in Arabidopsis thaliana under salt stress
Acet, Tuba; Kadıoğlu, Asım
SOS5 locus, encodes cell wall adhesion protein under salt stress conditions in plants, and it is required for normal cell expansion as well as for sustaining cell wall integrity and structure. However, it is still unknown how this gene locus-ABA cross-talk and interacts with the antioxidant mechanism under salt stress conditions. For this purpose, the study focused on mutant sos5-1 plant treated with ABA under NaCl stress and observed its growth and development as well as stomatal aperture, lipid peroxidation, proline, hydrogen peroxide (H2O2) and ABA contents, and some antioxidant enzyme activities. In addition, the expression levels of ABA related genes have been analysed by RT-PCR after stress application. According to findings, sos5-1 mutant plants treated with ABA under salt stress resulted in eliminated cellular damage compared to those which are solely exposed to salt stress; other observations include closing of stomata, decreased H2O2 content, increased amount of proline, and similarity with the wild type due to induced antioxidant enzyme activities. Besides, both ABA biosynthetic and inducible gene expressions of the mutant plant under salt stress were lower compared to the control, and catabolism gene expression was higher. As a result, SOS5 gene in synergy with ABA, scavenge the ROS by stimulating antioxidant system, leads to an increase in stress related gene expressions and thus contributes to salinity tolerance. This study is significant in the way that it shows how SOS5 gene locus, under salt stress conditions, interacts with antioxidant system in sustaining cell wall integrity.