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- MakaleAçık Erişim2022Investigation of the effects of different extraction methods on some biological activities of Dactylorhiza romana subsp. georgica (Klinge) Soó ex Renz & Taubenheim
Bozkir, Buğra; Acet, Tuba; Özcan, Kadriye
Dactylorhiza romana subsp. georgica is found in Anatolia and is traditionally used by local people both as food and to alleviate various ailments. Although various biological activities of the plant have been partially investigated, these properties have not been comprehensively revealed in the light of scientific data. In this study, it was aimed to elucidate the antioxidant, antimicrobial, enzyme inhibition (alpha amylase, alpha glucosidase, and tyrosinase) and DNA protective activity and phenolic content of the extracts obtained from orchid tubers using different extraction methods. For this, the fresh and dry tubers of the plant were extracted with ethanol at different times in an ultrasonic water bath, shaker and their combinations with each other. ABTS, DPPH, and FRAP methods were used to determine the antioxidant properties of plant tubers. In addition, the phosphomolybdate method was used to determine the total antioxidant capacity. The spectrophotometric method was preferred to reveal the enzyme inhibition activity. The antimicrobial activity of the extracts was investigated by microdilution method. In addition, the phenolic content of the plant was analysed by HPLC. The DNA protective activity of the extracts was determined by using the pUC 19 plasmid. According to the results obtained, it was revealed that each extract exhibited various biological activities. According to the HPLC results, the highest phenolic component in the extracts was determined as p-hydroxybenzoic acid. As a result, it was observed that the biological activities of orchid tubers changed according to different extraction methods. Accordingly, due to the high biological activities of orchid tubers, they have a high potential for use in the food and pharmaceutical industries.
- MakaleYalnız künye2020Phytochemical profile and biological activity of a therapeutic orchid from Anatolia: Dactylorhiza romana subsp. georgica
Kotiloğlu, Deniz; Acet, Tuba; Özcan, Kadriye
In Anatolia, the tubers of Dactylorhiza romana subsp. georgica orchid are consumed as food and frequently used in folk medicine to alleviate the symptoms of some disorders. However, these properties have not been scientifically studied until the present day. In this research, we aimed to identify phenolic profile of the orchid tubers and determine their several features and functions. Among these, there are the antioxidant and antimicrobial effects and the inhibitory effect against diabetes related enzymes (α-amylase and α-glucosidase). In this sense, total phenolic content (Folin–Cicalteu assay), total antioxidant capacity (Phosphomolybdate method), antioxidant (ABTS and DPPH assays) and enzyme inhibition effects of tuber extracts obtained with different solvents (chloroform, ethyl acetate, methanol, ethanol, or hexane) were determined by using spectrophotometric methods, and the phenolic profile was also analysed by HPLC. In addition, antimicrobial activity of the extracts against 3 g (+) and 3 g (−) bacteria and 2 forms of yeasts was determined by disc diffusion and micro dilution methods. Based on our findings, the total phenolic content and antioxidant properties of ethyl acetate extract were considerably higher than the others. The most abundant among the screened phenolic standards was found to be benzoic acid. The highest α-amylase and α-glucosidase enzyme inhibition was measured in methanol and chloroform extracts, respectively. The highest antibacterial (B. cereus) and anticandidal (C. tropicalis and C. albicans) activities were detected in the chloroform and ethyl acetate extracts, respectively. In short, it was clearly seen that the D. romana subsp. georgica tuber had a great potential for being used in food and drug industries as a source of bioactive compounds.