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- MakaleAçık Erişim2025Scopus SJRQ2The biological activity and phenolic content of endemic Astragalus baibutensis
Ozcan, Kadriye; Acet, Tuba; Yiğittürk, Gürkan
Astragalus baibutensis, is a plant endemic to Turkey, belonging to Fabaceae family, that is used as a folk medicine and food in this country. The purpose of this study is to find out the biological activities such as antioxidant, antimicrobial, enzyme inhibitory and DNA protective activity and also analyse the phenolic contents of the plant. To determine of antioxidant activity ABTS and DPPH assays were performed. Disc diffusion and microdilution methods were used to evaluate the antimicrobial activity. Additionally, enzyme inhibitory activities such as α-amylase, α-glucosidase, and tyrosinase were determined by using spectrophotometric methods. DNA protective activity was detected using pUC19 Plazmid DNA. The antiproliferative effect on SKOV-3 human ovarian cancer cells was examined by WST-8 assay. In addition, the phenolic contents were analysed by using HPLC. The biological activity of ethyl acetate extract of the root was found to be higher than those of other extracts. This extract had the highest amount of apigenin as phenolic compound. On the other hand, extracts showed moderate inhibition against the tested microorganism and antiproliferative properties in a concentration-dependent manner toward ovarian cancer cells. According to the results, Astragalus baibutensis has the potential for pharmaceutical and food additive usage.
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İncele - MakaleAçık Erişim2022Scopus SJRQ2Investigation of the effects of different extraction methods on some biological activities of Dactylorhiza romana subsp. georgica (Klinge) Soó ex Renz & Taubenheim
Bozkir, Buğra; Acet, Tuba; Özcan, Kadriye
Dactylorhiza romana subsp. georgica is found in Anatolia and is traditionally used by local people both as food and to alleviate various ailments. Although various biological activities of the plant have been partially investigated, these properties have not been comprehensively revealed in the light of scientific data. In this study, it was aimed to elucidate the antioxidant, antimicrobial, enzyme inhibition (alpha amylase, alpha glucosidase, and tyrosinase) and DNA protective activity and phenolic content of the extracts obtained from orchid tubers using different extraction methods. For this, the fresh and dry tubers of the plant were extracted with ethanol at different times in an ultrasonic water bath, shaker and their combinations with each other. ABTS, DPPH, and FRAP methods were used to determine the antioxidant properties of plant tubers. In addition, the phosphomolybdate method was used to determine the total antioxidant capacity. The spectrophotometric method was preferred to reveal the enzyme inhibition activity. The antimicrobial activity of the extracts was investigated by microdilution method. In addition, the phenolic content of the plant was analysed by HPLC. The DNA protective activity of the extracts was determined by using the pUC 19 plasmid. According to the results obtained, it was revealed that each extract exhibited various biological activities. According to the HPLC results, the highest phenolic component in the extracts was determined as p-hydroxybenzoic acid. As a result, it was observed that the biological activities of orchid tubers changed according to different extraction methods. Accordingly, due to the high biological activities of orchid tubers, they have a high potential for use in the food and pharmaceutical industries.
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