Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleYalnız künye2025Phytochemical Profile, Antioxidant Capacity, Acetylcholinesterase, and Butyrylcholinesterase Enzyme Inhibition Properties of Myosotis amoena Boiss
Yapici, Ismail; Kayaoglu, Esma; Izol, Ebubekir; Yilmaz, Mustafa Abdullah; Behçet, Lutfi; Cakir, Oguz; Gülçin, Ilhami
Myosotis amoena Boiss. (Turkish name: Ho & scedil; boncukotu) is a perennial herbaceous plant that produces blue flowers during the spring. Phytochemicals, which are secondary metabolites distributed across plants, fungi, microorganisms, and even animal tissues, play crucial roles in health through their antioxidant and bioactive properties. Their antioxidant capacity largely arises from hydroxyl groups and aromatic ring structures that stabilize free radicals via electron delocalization. Enzyme inhibition is a therapeutic strategy in several diseases, including Alzheimer's disease (AD), where acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitors are employed to manage symptoms. This study investigated the phytochemical composition (via LC-MS/MS), total phenolic content, antioxidant capacity, and AChE/BChE inhibitory effects of the ethanol extract of M. amoena using four in vitro methods. The CUPRAC assay demonstrated that M. amoena (1.070) exhibited higher antioxidant activity than Trolox (0.894), although its activity in FRAP, ABTS, and Fe3+ reduction assays was lower compared to standard antioxidants. The extract contained 53.03 mg GAE/g extract of total phenolics, with rosmarinic acid being the predominant compound (15.27 mg/g). Furthermore, the extract strongly inhibited AChE and BChE, with IC50 values of 3.9 and 19.8 mu g/mL, respectively. These findings highlight, for the first time, the significant bioactive potential of M. amoena as a natural source of antioxidants and cholinesterase inhibitors, supporting its prospective use in the prevention or management of neurodegenerative conditions.
- MakaleAçık Erişim2025Screening of Chemical Profile by LC–MS/MS and Assessment of Biological Properties of Achillea sintenisii Hub. Mor. From Turkey
Köksal, Ekrem; Altay, Ahmet; Altın, Sevgi; Alp, Cemalettin; Ulutaş, Yakup; Şimşek, Samed; Karakuş, Sinem; Akyüz, Sümeyye; Aygül, İmdat; Akşit, Hüseyin
The aim of this study was to determine the secondary metabolites of the n-hexane, ethyl acetate, methanol, and water extracts of Achillea sintenisii Hub. Mor. and to ascertain their divergent biological properties. LC–MS/MS analysis was performed to identify the bioactive compounds. The anticancer properties of the samples were tested against human breast (MCF-7 and MDA-MB-453) and colon (HT-29) cancer cell lines by XTT assay. The anti-Alzheimer's studies were performed using Ellman's assay. The antimicrobial studies were carried out by Kirby–Bauer disc diffusion method. The antioxidative potential of the samples was evaluated on the basis of 2,2-diphenyl-1-picrylhydrazyl scavenging and ferric reducing antioxidant power assays, LC–MS/MS analysis revealed 27 phytochemicals, including high concentrations of quinic acid, gentisic acid, chlorogenic acid, luteolin, cynaroside, and caffeic acid. Ethyl acetate extract exhibited the most potent cytoxicity against MDA-MB-453 cells, with an IC50 value of 83.05 µg/mL. All extracts showed stronger inhibitory activity against acetylcholinesterase and butyrylcholinesterase compared to tacrine. The n-hexane, ethyl acetate, and methanol extracts exhibited considerable antimicrobial activity against Enterococcus faecalis, approaching the level of vancomycin. Methanol extract possessed the highest DPPH scavenging and ferric reducing antioxidant power. These findings render A, sintenisii a promising source for the pharmaceutical, cosmetic, and food industries.