Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2015Effects of Turkish propolis on expression of hOGG-1 and NEIL-1
Turan, İbrahim; Değer, Orhan; Aliyazicioğlu, Yüksel; Demir, Selim; Kilinç, Kağan; Sümer, Ayşegül
Background/aim: Propolis is a bee product with antioxidative, antimutagenic, and other beneficial properties, and it is used as a natural drug. It is rich in polyphenolic compounds. Its composition varies depending on the particular geographical region. Oxidative stress is caused by an imbalanced free radical production and antioxidant system. The effects of flavonoids on the expression of DNA repair enzymes have been examined previously; however, no study has investigated the effects of propolis. This study investigated the effects of ethanolic extracts of Turkish propolis (EEP) on the expression of DNA repair enzymes. Materials and methods: The effects of EEP and tertiary-butyl-hydroperoxide (t-BHP) on cell viability were determined using MTT. DNA damage was determined using comet assay. mRNA expression of target enzymes was detected using RT-PCR. Results: According to the cytotoxicity analysis, after a recovery time of 4 h, appropriate damage agent t-BHP and optimum EEP concentrations were 300 µM and 200 µg/mL, respectively. 8-Oxoguanine-glycosylase (hOGG-1) and endonuclease-VIII-like-1 (NEIL-1) expressions increased in the positive control group (t-BHP alone) and the study group (t-BHP+EEP). Maximum increase in NEIL-1 expression was at hour 12 in the positive control group and at hour 8 in the study group. Conclusion: EEP can be considered as a potential source of functional food and pharmaceutical agents.
- MakaleAçık Erişim2015Evaluation of oxidative stress balance in acute deep vein thrombosis; [Akut derin ven trombozunda oksidatif stres dengesinin değerlendirilmesi]
Menteşe, Ümit; Turan, İbrahim; Doğan, Süheyla; Sümer, Ayşegül; Coşkun, Ceyhun; Alver, Ahmet; Doğan, Orhan Veli; Menteşe, Seda Öztaş; Menteşe, Ahmet
Objective: The purpose of this study was to show the association between oxidative stress and deep venous thrombosis (DVT) by determining total oxidant status (TOS), total antioxidant status (TAS) and oxidative stress index (OSI) values in patients with DVT and to take precautionary measures to balance oxidative status. Methods: TOS, TAS and OSI levels in serum from 52 individuals with DVT and 45 without DVT were compared. Results: TOS and OSI levels were significantly higher in patients with DVT compared with the control group (p<0.0001), but no difference was observed in TAS levels (p=0.118). The patient group was divided into two subgroups, idiopathic and secondary DVT. TOS and OSI levels were significantly higher patients with both idiopathic DVT and secondary DVT compared to the control group (p<0.0001). TAS levels were significantly higher in patients with idiopathic DVT than in the control group, but no difference was observed in the patients with secondary DVT. Conclusion: Specificity and sensitivity values obtained from the receiver operating characteristic (ROC) curve show that TOS and OSI are both markers that can be used in the diagnosis of DVT. Also, determination of oxidative stress markers may guide clinicians to take necessary precautions to balance oxidative stress in the patients.
- MakaleYayıncıda açık erişim2013Serum anti-carbonic anhydrase I and II antibodies and polycystic ovary syndrome; [Serum anti-karbonik anhidraz I ve II antikorları ve polikistik over sendromu]
Menteşe, Ahmet; Güven, Süleyman; Sümer, Ayşegül; Turan, Ibrahim; Demir, Selim; Karahan, Süleyman Caner; Alver, Ahmet
Aim: The aim of this study is to investigate anti-carbonic anhydrase antibodies (anti-CA I and CA II antibodies) in the sera of women with polycystic ovary syndrome (PCOS). Methods: In this study serum anti-CA I and II antibody levels of age and BMI matching fifty women with PCOS and fifty women without PCOS on day three of menstrual cycle were assessed with an ELISA method previously developed by Hosoda and modified by Alver et al. Results: The mean serum anti-CA I antibody levels were significantly higher in women with PCOS and anti-CA II antibody levels were not significantly different in women with PCOS compared with control subjects. For serum anti-CA I antibody, the absorbance higher than 0.484 (mean + 3SD of control subjects) was taken as positive, Anti-CA I antibody was detected in 13 of 50 patients with PCOS (26%). Considering serum anti-CA II antibody, the absorbance higher than 0.654 (mean + 3SD of control subjects) was taken as positive. Anti--CA II antibody was detected in 2 of 50 patients with PCOS (4%). All patients with positive anti-CA II antibody also had positive anti-CA I antibody. Autoantibodies specifically reactive to CA I were found to be present at a higher frequency than CA II in the serum of subjects with PCOS in the present study. Conclusion: The results may suggest that autoimmune responses against CA I may be involved in the pathogenesis of PCOS.