Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2025Assessment of antioxidant, antimicrobial, antibiofilm, carbonic anhydrase and α-glucosidase inhibitory activities of Alchemilla vulgaris L. extracts
Düzgün, Azer Özad; Akar, Zeynep; Kardil, Ugur
This study was to investigate the biological properties (antioxidant, enzyme inhibitory and antimicrobial activity) of water extract (WE), 50:50% water:ethanol extract (WEE) and ethanol extract (EE) of aerial parts of Alchemilla vulgaris L. which was traditionally used to alleviate and treat many diseases. The WEE extract exhibited the highest total phenolic content (TPC), with a value of 612 µg/mL GAE, while the EE extract demonstrated the highest total flavonoid content (TFC), with a value of 0.086 µg/mL GAE. Also, the data demonstrated that the WEE extract exhibited the highest radical scavenging capacities and antioxidant activities, exhibiting 0.0056, 0.0028 mg/mL SC50 and 267, 0.081 μM TEAC, as determined by the DPPH, ABTS, FRAP and CUPRAC assays, respectively. The WEE extract was found to be an effective bovine carbonic anhydrase (BCA) and α-glucosidase inhibitor with an IC50 values of 0.0628 and 0.0535 mg/mL, respectively. The EE extract showed activity against both Gram-negative and Gram-positive bacteria used in the study. EE extract has the highest activity against Yersinia pseudotuberculosis and the lowest activity against Streptococcus pyogenes. Furthermore, the extracts significantly reduced the biofilm ability of Acinetobacter baumannii isolate, which has strong biofilm ability. EE and WE reduced the biofilm formation capacity of the strain to a weak level, while WEE extract reduced it to a moderate level. The results suggest that EE has a significant effect in combating antibiotic resistance, while WEE has high antioxidant activity and good inhibitory effect against BCA and α-glycosidase enzymes. In addition, the results revealed that solvent extracts with different solvent composition and polarity may have different effects on diverse bioactivity tests.
- MakaleAçık Erişim2024Investigation of antibiofilm and biological activities of Vaccinium arctostaphylos L.
Düzgün, Azer Özad; Akar, Zeynep; Kardil, Ugur
This study investigated the total phenolic and flavonoid contents and the antioxidant, antimicrobial, antibiofilm and α-glucosidase inhibitory activities of methanol extract from Vaccinium arctostaphylos L. leaf and fruit parts. The highest antioxidant activity with 53 µM TEAC and 0.0084 mg/mL SC50 using the ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays, respectively determined in leaf part. The data indicated that the leaf of the plant had the higher total phenolic content (49 µg/mL GAE) and total flavonoid content (0.071 µg/mL QAE) compared to fruit. The α-glucosidase enzyme activity of the leaves (0.179 mg/mL IC₅₀) was observed to be higher than that of the fruits (0.386 mg/mL IC₅₀). The MIC values of the leaf and fruit parts of blueberry were 6.25 mg/ml and 3.125 mg/ml, respectively. The results of this study indicate that the leaf extract was found to significantly reduce the biofilm-forming capacity of the Acinetobacter baumannii isolate by approximately 3-fold, whereas the fruit extract was observed to have only a marginal effect, reducing the biofilm-forming capacity by approximately 1.4-fold. The effects of plant extracts on microbial biofilms may be examined with a view to combating antibiotic resistance. Also results suggesting that it might be an effective and safety medical plant to prevent or treat diseases associated with oxidative damage and bacterial infections. Consequently, further research on natural substances may lead to the development of treatment options.
- MakaleAçık Erişim2024Vitis Labrusca L. Kabuk Ekstraktlarının Antibiyofilm, Antidiyabetik ve Antioksidan Potansiyelleri
Düzgün, Azer Özad; Akar, Zeynep; Kardil, Ugur
Bu çalışmada, Vitis labrusca L. bitkisinin kabuk kısımlarından elde edilen metanol, 50:50% metanol:su ve su ekstraktlarının antioksidan, antimikrobiyal, antibiyofilm ve α-glukozidaz inhibitör aktiviteleri ile toplam fenolik ve flavonoid içerikleri incelenmiştir. 50:50% metanol:su özütü, sırasıyla FRAP ve DPPH• radikal süpürücü analizleriyle belirlendiği üzere, 153 μM TEAC ve 0,0947 mg/mL SC50 ile en yüksek antioksidan aktiviteyi sergilemiştir. 50:50% metanol:su ekstraktı, sırasıyla 141 μg/mL GAE ve 0,103 mg/mL IC₅₀ değeri ile diğer çözücü ekstraktları arasında en yüksek toplam fenolik içeriği ve α-glukozidaz enzim inhibisyonunu sergilemiştir. Metanol, su ve 50:50% metanol:su ekstraktlarının MİK değerleri, klinik antibiyotik direnci olan Acinetobacter baumanii' ye karşı sırasıyla 6.25, 25 ve 12.5 mg/mL olarak belirlenmiştir. Metanol, su ve %50:50 metanol:su ekstraktlarının Acinetobacter baumannii izolatının biyofilm oluşturma kapasitesini sırasıyla yaklaşık 1.7, 1.6 ve 1.3 kat azalttığı bulunmuştur. Bulgular, 50:50% metanol:su ekstraktının en yüksek fenolik içerik, antioksidan aktivite ve α-glukozidaz inhibisyonu sergilediğini, metanol ekstraktının ise en belirgin antimikrobiyal ve antibiyofilm aktivitelerini gösterdiğini ortaya koymuştur. Araştırmamızın sonuçları, çeşitli çözücülerin ve bunların ilgili bileşimlerinin kullanımının biyolojik aktivitede önemli farklılıklar yaratma kapasitesine sahip olduğunu göstermektedir. Bu durum, V. labrusca kabuk bileşenlerinin oksidatif stres ve bakteriyel enfeksiyonlarla ilgili rahatsızlıkların ele alınmasında değerli bir yol olarak hizmet etme potansiyeline işaret etmektedir.