Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2025Screening of Chemical Profile by LC–MS/MS and Assessment of Biological Properties of Achillea sintenisii Hub. Mor. From Turkey
Köksal, Ekrem; Altay, Ahmet; Altın, Sevgi; Alp, Cemalettin; Ulutaş, Yakup; Şimşek, Samed; Karakuş, Sinem; Akyüz, Sümeyye; Aygül, İmdat; Akşit, Hüseyin
The aim of this study was to determine the secondary metabolites of the n-hexane, ethyl acetate, methanol, and water extracts of Achillea sintenisii Hub. Mor. and to ascertain their divergent biological properties. LC–MS/MS analysis was performed to identify the bioactive compounds. The anticancer properties of the samples were tested against human breast (MCF-7 and MDA-MB-453) and colon (HT-29) cancer cell lines by XTT assay. The anti-Alzheimer's studies were performed using Ellman's assay. The antimicrobial studies were carried out by Kirby–Bauer disc diffusion method. The antioxidative potential of the samples was evaluated on the basis of 2,2-diphenyl-1-picrylhydrazyl scavenging and ferric reducing antioxidant power assays, LC–MS/MS analysis revealed 27 phytochemicals, including high concentrations of quinic acid, gentisic acid, chlorogenic acid, luteolin, cynaroside, and caffeic acid. Ethyl acetate extract exhibited the most potent cytoxicity against MDA-MB-453 cells, with an IC50 value of 83.05 µg/mL. All extracts showed stronger inhibitory activity against acetylcholinesterase and butyrylcholinesterase compared to tacrine. The n-hexane, ethyl acetate, and methanol extracts exhibited considerable antimicrobial activity against Enterococcus faecalis, approaching the level of vancomycin. Methanol extract possessed the highest DPPH scavenging and ferric reducing antioxidant power. These findings render A, sintenisii a promising source for the pharmaceutical, cosmetic, and food industries.
- MakaleYalnız künye2021N epeta meyeri essential oil ameliorates fungal infection and the antioxidant response in grapevines (Vitis vinifera) infections by gray mold (Botrytis cinerea)
Karakuş, Sinem; Atici, Ökkeş; Köse, Cafer; Aydin, İhsan
The essential oil (EO) obtained from Nepeta meyeri Benth. used to struggle allelopathically with gray mold (Botrytis cinerea Pers.), which causes significant damage to vineyards (Vitis vinifera L. cv. Karaerik). The EO from N. meyeri was determined to contain 4aα, 7α, 7aα-nepetalactone (71.68%) at the highest rate when analyzed with GC/MS. Then, the doses (0.6, 0.8, and 1.0 µL/leaf) of 1 ppm EO solution were applied to the leaves together with the fungus inoculation (EO + F), as well as 1 day (EO + F1D) and 2 days (EO + F2D) before inoculation. The fungus application alone was evaluated as a control. Seven days after the inoculations, fungus development, lipid peroxidation level (as malondialdehyde), the contents of reactive oxygen species (ROS), photosynthetic pigments and soluble proteins, and the activities of antioxidant enzymes (superoxide dismutase, catalase, peroxidase, and glutathione reductase) were determined in the leaves. Findings revealed that EO + F1D among the applications and 1.0 µL/leaf among the EO doses significantly prevented fungus development on the leaf, compared to the control. Moreover, the same applications decreased ROS levels, such as H2O2 and O2·− and lipid peroxidation, while they increased antioxidant enzyme activities, as well as chlorophyll and soluble protein contents. Therefore, it can be suggested that the EO from N. meyeri has a potentially powerful usage as biofungicide in the allelopathic struggle against the infection of B. cinerea in vineyards.