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- MakaleAçık Erişim2026Scopus SJRQ1Isolation and Preliminary In Vitro Characterization of a Honey-Derived Bacillus licheniformis Isolate
Usta, Mehtap; Zengin, Kübra; Kara, Yakup; Nalçacıoğlu, Remziye; Demirbağ, Zihni; Yıldız, Oktay; Kolaylı, Sevgi
Background/Objectives: Despite its hostile physicochemical environment characterized by high osmotic pressure, acidic pH, and low water activity, honey serves as a unique ecological niche for resilient microorganisms. This study aimed to recover bacterial isolates from honeys of different botanical origins and to characterize the probiotic-associated properties of a honey-derived Bacillus licheniformis isolate. Methods: A total of 20 bacterial isolates were recovered from four honey samples, including two blossoms and two honeydew honeys. 16S rRNA gene sequence analysis showed that the recovered isolates had taxonomic assignments consistent with Bacillus licheniformis. Because 16S rRNA sequencing does not provide sufficient resolution to establish strain-level diversity or clonality, one isolate, designated Blhoney, was selected for subsequent molecular and functional characterization. Results: In vitro assays revealed that Blhoney exhibits robust tolerance to simulated gastrointestinal stress, including extreme acidity (pH 1–3), bile salts (0.3–1%), pepsin, and pancreatin, maintaining viability for up to 24 h. Phenotypic antimicrobial testing demonstrated measurable inhibition zones for erythromycin, kanamycin, chloramphenicol, ampicillin, tetracycline, gentamicin, streptomycin, and spectinomycin, whereas no measurable inhibition zones were observed for penicillin G, rifampicin, ciprofloxacin, or ceftazidime. However, MIC determination and standardized breakpoint interpretation were not performed; therefore, these findings were interpreted solely as preliminary phenotypic inhibition-zone observations. Although the isolate did not display direct antagonistic activity against the tested bacterial pathogens under experimental conditions, its persistence under the tested gastrointestinal stress conditions supports further investigation of its probiotic-associated characteristics. Conclusions: Overall, these findings indicate that the honey-derived Blhoney isolate exhibits preliminary probiotic-associated characteristics under the tested in vitro conditions. However, further strain-level genomic and safety characterization is required before its potential probiotic application can be established.
- MakaleYalnız künye2023Determination of cytotoxic, anti-acetylcholinesterase and antioxidant activity of some medicinal Artemisia spp.
Gıdık, Betül; Kara, Yakup; Akar, Zeynep; Demir, Elif Ayazoglu; Can, Zehra
The study aims to investigate the antioxidant activities, phenolic compounds, acetylcholinesterase enzyme inhibition and cytotoxic effects of two different Artemisia spp. (Artemisia dracunculus L. and Artemisia dracunculoides Pursh) cultivated in Türkiye organically, for the first time. The total phenolic and total flavonoid contents of the plants were determined spectrophotometrically while antioxidant activity DPPH•, CUPRAC, and FRAP was determined using the colorimetric method. And also, acetylcholinesterase in vitro enzyme inhibition activity and anti-cancer activity against human melanoma (VMM917, CRL-3232), lung carcinoma (A549, CCL-185), and normal human fibroblast (hGF, PCS-201-018) cells were studied. Total phenolic (225-324 μg GAE/mL extract) and total flavonoid contents (0.066-0.085 μg QE/mL extract), antioxidant activity Color Scavenging Concentration 50% (DPPH• (CSC50: 1.371-1.655 mg/mL), CUPRAC (0.246-0.344 μM CTEAC) and FRAP (462.133-726.661 μM CTEAC)). A. dracunculus and A. dracunculoides extracts inhibited 40.09 ± 0.65%, and 39.48 ± 3.68% of acetylcholinesterase activity. It was determined that demonstrated the selective effect of A. dracunculus and A. dracunculoides on the cytotoxicity of A549 and hGF cells. According to our results, both tarragon plant species, it may be said that it is not only consumed as food but also used for therapeutic purposes will be beneficial for health.
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