Kurt, Akif Hakan; Paksoy, Zahid; Ergün, Yusuf

Enrofloxacin is a broad spectrum antibiotic used to treat bacterial infections. The aim of present study was to investigate the effect of enrofloxacin on Prostaglandin F2 alpha (PGF2 alpha) induced postpartum canine myometrium in vitro. Uterine tissues were obtained from bitches undergoing ovariohysterectomy in the postpartum period. Isolated myometrium strips suspended in organ bath filled with Kumagai solution and continuously gassed with 95% oxygen and 5% carbon dioxide (28°C). At the end of equilibration period, PGF2 alpha was added to the organ bath and the myometrial strips were observed to be contracted. Then the organ bath were administered solvent (n = 6), 2 mM (n = 6) and 5 mM (n = 10) enrofloxacin and % contraction was monitored. PGF2 alpha was given a second time and the contractions wererecorded after incubation with enrofloxacin. Solvent inhibited significantly myometrial contractions and enrofloxacin treated group showed a biphasic effect. The contractile response was significantly inhibited by 5 mM enrofloxacin compared to the control group. Our results show that high-dose enrofloxacin had inhibitory effects on the canine myometrial contractions. For this reason, enrofloxacin should be used combine with uterotonic agents in the metritis treatment.