Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleYalnız künye2018Copper, Zinc, Magnesium and Calcium Levels in Royal Jelly That Produced in Bursa Province
Uçar, Meltem; Bulut, Volkan Numan; Vanizor Kural, Birgül; Kopuz, Müge; Değer, Orhan; Aliyazıcıoğlu, Rezzan; Cengiz, Sevil
Aim: Royal jelly is a bee product that secreted from glands of young worker bees and used resource of food for larvae and queen honeybee. Royal jelly which is very important for physiological development of queen honeybee and larvae, also used by people due to the nutritional properties and positive effects on health. Royal jelly contains water, lipid, protein, carbohydrate, amino acid, short peptide, vitamin, phenolic compounds, trace element and minerals. It was suggested that composition of royal jelly varies depending on the geographical structure of the zone, climate, flora that it is produced, storage condition and bees race. There are also publications which suggest that mineral content of royal jelly is independent of geographical structure and climate, but only varies depending on the harvesting time. There are not so many study related with chemical composition and biological properties of royal jelly that is produced in Turkey. In this study, it was aimed to determine some element levels in royal jelly samples that obtained from Bursa province, Mustafakemalpaşa district. Material and Methods: Element levels of copper (Cu), zinc (Zn), magnesium (Mg) and calcium (Ca) determined by Atomic Absorption Spectrophotometer (AAS) (n=3). For each element, the results were exhibited as mean±standard deviation. Results: The amount of Cu was determined 3.25±0.05 mg/kg, the amount of Zn was 23.87±1.33 mg/kg, the amount of Mg was 794.33±38.44 mg/kg and the amount of Ca was 250.87±24.49 mg/kg in Bursa royal jelly. Conclusion: Element levels of Bursa royal jelly are at a level that can contribute to daily mineral requirement. However, there is a need for further in vivo studies on the availability of mineral additives and their therapeutic utility.
- MakaleAçık Erişim2016Effect of Turkish pollen and propolis extracts on caspase-3 activity in myeloid cancer cell lines
Uçar, Meltem; Değer, Orhan; Gerigelmez, Asuman Yiğit; Cengiz, Sevil; Barlak, Yaşam; Ovalı, Ercüment
Purpose: To investigate the apoptosis-inducing capacity of dimethyl sulfoxide (DMSO) extracts of bee pollen and propolis in HL-60 Myeloid Cancer Cell Lines. Methods: DMSO extracts of pollen and propolis were incubated separately with HL-60 cells, and caspase-3 activity evaluated. In order to determine the cell cycle characteristics of HL-60 cells with and without extracts of pollen and propolis, the cells were analysed using flow cytometry. Results: The DMSO extract of propolis (0.5 mg/mL) increased apoptosis from undetectable levels to 60.1%, while maintaining cell viability. The DMSO extract of pollen (2 mg/ml) increased apoptosis from undetectable levels to 52.2% while decreasing cell viability by 62%. Caspase-3 activity in HL-60 cells incubated with DMSO extracts of pollen and propolis were 3.6-to 12-fold higher than in controls. Conclusion: Turkish pollen and propolis individually increase apoptosis and the activity of caspase-3 in HL-60 cells. This finding indicates that bee products may have beneficial effects in the treatment of cancer.
- MakaleAçık Erişim2015Effects of Turkish propolis on expression of hOGG-1 and NEIL-1
Turan, İbrahim; Değer, Orhan; Aliyazicioğlu, Yüksel; Demir, Selim; Kilinç, Kağan; Sümer, Ayşegül
Background/aim: Propolis is a bee product with antioxidative, antimutagenic, and other beneficial properties, and it is used as a natural drug. It is rich in polyphenolic compounds. Its composition varies depending on the particular geographical region. Oxidative stress is caused by an imbalanced free radical production and antioxidant system. The effects of flavonoids on the expression of DNA repair enzymes have been examined previously; however, no study has investigated the effects of propolis. This study investigated the effects of ethanolic extracts of Turkish propolis (EEP) on the expression of DNA repair enzymes. Materials and methods: The effects of EEP and tertiary-butyl-hydroperoxide (t-BHP) on cell viability were determined using MTT. DNA damage was determined using comet assay. mRNA expression of target enzymes was detected using RT-PCR. Results: According to the cytotoxicity analysis, after a recovery time of 4 h, appropriate damage agent t-BHP and optimum EEP concentrations were 300 µM and 200 µg/mL, respectively. 8-Oxoguanine-glycosylase (hOGG-1) and endonuclease-VIII-like-1 (NEIL-1) expressions increased in the positive control group (t-BHP alone) and the study group (t-BHP+EEP). Maximum increase in NEIL-1 expression was at hour 12 in the positive control group and at hour 8 in the study group. Conclusion: EEP can be considered as a potential source of functional food and pharmaceutical agents.
- MakaleAçık Erişim2014Is the combined use of insulin resistance indices, Including adipokines, more reliable in metabolic syndrome?
Kural, Birgül; Değer, Orhan; Erem, Cihangir; Balaban Yücesan, Fulya; Aliyazicioğlu, Rezzan; Barlak, Yaşam
Background/aim: To determine the levels of adipokines (leptin, adiponectin, resistin, and visfatin) and the indices of insulin sensitivity/ resistance, and to examine the relationship among them in patients with metabolic syndrome (MetS).; Materials and methods: The study groups included 45 subjects with MetS (31 women/14 men), and 45 sex- and age-matched non-MetS healthy volunteers (31 women/14 men). The levels of adipokines were determined by enzyme-linked immunosorbent assay.; Results: The levels of leptin and visfatin were significantly higher in the MetS than in the non-MetS subjects (P < 0.01). There was no difference in adiponectin levels in subjects with and without MetS (P = 0.052). Similarly, resistin did not show any statistically significant difference. A statistically significant positive correlation of leptin with insulin levels was observed, while negative correlations of visfatin levels with age, and resistin levels with the ratio of adiponectin to leptin, were found in the MetS (P < 0.05). The combination of adipokines, insulin resistance-sensitivity parameters, and MetS criteria parameters gave more signifcant differences than a single parameter.; Conclusion: Since the parameters mentioned above might affect, interact with, and/or interfere with each other, the combinations of these parameters might give more reliable results to evaluate the insulin resistance/sensitivity in MetS patients.