Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2026Phenolic and antioxidant assessment of the flowers, stems, leaves, and fruits of Rosa pimpinellifolia and Rosa canina at different growth time
Öz, Mehmet; Okan, Onur Tolga; Baltaci, Cemalettin
Natural plants are abundant in their native habitats and are traditionally used to treat a variety of health conditions. The Rosa genus stands out as an important species rich in bioactive compounds. This study, conducted in Türkiye, aimed to analyse the antioxidant and phenolic profiles of methanolic extracts obtained from different parts of Rosa canina (R.c.) and Rosa pimpinelifolia (R.p.) collected in 2021 and 2022. The antioxidant activity values of R.c. collected in 2021 and 2022 were found to be between 5 102.31–46 396.36 mmol Fe(II)/100 g in ferric reducing antioxidant power (FRAP), 141.92–377.54 mg ascorbic acid equivalents (AA)/kg in total antioxidant capacity (TAC), 2.18–24.68 µg/g in 2,2-diphenyl-1-picrylhydrazyl [DPPH (EC50)] and 34.31–75.77% in DPPH (% inhibition). Antioxidant activity of R.p. collected in 2021 and 2022 ranged from 17 258.44–71 903.20 mmol Fe(II)/100 g, 271.69–479.19 mg AA/kg, 1.02–8.32 µg/g and 50.98–85.57% for FRAP, TAC, DPPH (EC50) and DPPH (% inhibition), respectively. The R.p. extracts generally exhibited higher antioxidant activity than those of R.c. Considering the total identified phenolic compounds achieved from different plant parts of rosehip for both years, the highest values of total phenolic compounds were determined in R.c. leaves (222.08 mg/100 g) in 2021, and R.p. leaves (5 465.95 mg/100 g) in 2021.
- MakaleAçık Erişim2026Chemical Profiling of Stachys cretica subsp. anatolica Rech. f. (Endemic) Essential Oils and their Methanol Extracts with Evaluation as Enzyme Inhibitors, Antioxidant, and Antimicrobial Agents
Öz, Mehmet; Fidan, Muhammed Said; Baltacı, Cemalettin; Akmeşe, Osman; Sefalı, Abdurrahman; Aygül, İmdat
Enzyme inhibition activities, phenolic compounds, antioxidant activities, bioactive compounds, antimicrobial activities, and chemical components of essential oil and methanol extracts obtained from the aerial parts of S. cretica subsp. anatolica were investigated. The main phenolic compounds of aerial parts were catechin, oleuropein, and epicatechin. The determined enzyme inhibitor activities highlight the potential of S. cretica subsp. anatolica as a source of bioactive compounds, particularly for carbonic anhydrase and cholinesterase inhibition. The essential oil and methanol extract exhibited remarkable activities against CA-II, AChE, and BChE, although they were less potent than standard inhibitors. The essential oils generally showed stronger antimicrobial activity compared to the 30% methanol extracts across most bacterial and fungal strains, as evidenced by minimum lethal concentration (MLC) and lower minimum inhibitory concentration (MIC) values and larger inhibition zones. Chloramphenicol used alone exhibited the highest antimicrobial efficacy, with the lowest MIC and MLC values and the largest inhibition zones. The essential oils of S. cretica subsp. anatolica were determined as esters, oxygenated sesquiterpenes, and aldehydes in aerial parts. The main components were found to be hexahydrofarnesyl acetone in the aerial parts.
- MakaleAçık Erişim2026Chemical Profiling and Biological Activities of Methanol Extracts and Essential Oils From Ferulago Platycarpa Boiss. & Balansa (Endemic): Enzyme Inhibition, Antioxidant, and Antimicrobial Properties
Aygül, İmdat; Öz, Mehmet; Fidan, Muhammed Said; Baltacı, Cemalettin; Akmeşe, Osman; Sefalı, Abdurrahman
This study aimed to evaluate the phytochemical composition and biological activities of Ferulago platycarpa methanol extract (ME) and essential oil (EO). The EO was obtained via hydrodistillation and analyzed by GC-MS, revealing sesquiterpene hydrocarbons as the dominant class, with caryophyllene (65.8%) and α-pinene (9.65%) as the major constituents. The methanol extract was subjected to LC-MS/MS analysis, which identified eleven phenolic compounds, with chlorogenic acid as the most abundant. The extract showed significantly higher total phenolic (1196.22±11.64 mg GAE/100g) and antioxidant (1870.00±17.69 mg QEE/100g) contents compared to the ME. In vitro enzyme inhibition assays demonstrated that the methanol extract exhibited potent inhibitory activity against carbonic anhydrase II (CA-II, ICSO= 0.023 pg/mL), acetylcholinesterase (AChE, ICSO= 110 pg/mL), and butyrylcholinesterase (BChE, IC50= 390 pg/mL). In contrast, the EO showed higher inhibition against α-amylase (IC50= 5920±10.45 pg/mL) and BChE (IC50= 1.32±0.65 pg/mL), while its α-glucosidase showed no inhibition. Antioxidant assays indicated superior activity for the methanol extract compared to the EO. Furthermore, antimicrobial testing revealed that the EO demonstrated broader and more effective antimicrobial action, exhibiting lower MIC and MBC values against several bacterial and fungal strains. Collectively, these results highlight F. platycarpa as a valuable source of bioactive compounds with promising applications in antidiabetic, neuroprotective, antioxidant, and antimicrobial therapies.
- MakaleAçık Erişim2025Chemical composition of essential oils and comparative analysis of enzyme inhibitory, antioxidant and antimicrobial activities in essential oils of endemic Gelasia sericea (Aucher ex DC.) Zaika, Sukhor. & N. Kilian
Fidan, Muhammed Said; Baltacı, Cemalettin; Akmeşe, Osman; Sefalı, Abdurrahman; Aygül, İmdat; Öz, Mehmet
In this study, essential oil from the aerial parts of Gelasia sericea (Aucher ex DC.) Zaika, Sukhor. & N. Kilian was extracted via hydrodistillation using a Clevenger apparatus. GC-MS/FID analysis determined aldehydes (28.31%) as the dominant group, with eucalyptol (8.71%) as the main constituent. Antioxidant activities were exhibited using DPPH, ABTS, and FRAP assays, with the essential oil demonstrating relatively higher antioxidant properties. The essential oil also showed higher levels of bioactive components, including total antioxidant capacity (160.26 mg AA eq./100 mL), total phenolic content (21.57 mg GA eq./100 mL), and total flavonoid content (14.85 mg QE eq./100 mL). While G. sericea essential oil demonstrated some antioxidant activity, it was significantly lower compared to the reference standard ascorbic acid (AA). The antimicrobial results showed relatively higher antimicrobial activity compared to most bacterial and fungal strains, as evidenced by lower MIC and MBC values and larger inhibition zones. Nevertheless, chloramphenicol (CH) and nalidixic acid (NA) continually showed superior antimicrobial efficacy, with the lowest MIC and MBC values and the largest inhibition zones. The essential oil also demonstrated potent enzyme inhibitory activity, especially against CA-II, AChE, and BChE, with the lowest IC50 value observed for CA-II, highlighting its potential as a potent enzyme inhibitor. However, the essential oil exhibited comparatively greater inhibition against AChE, BChE and α-amylase but was less effective overall. These findings suggest that the G. sericea essential oil has important antioxidant potential and enzyme inhibitory activity, while the essential oil shows varying efficacy across different enzymes.
- MakaleYalnız künye2025Characterization of Aroma Components by HPLC-DAD and HS-SPME/GC-MS of Whortleberry and Bilberry Fruits (Vaccinium arctostaphylos L. and Vaccinium myrtillus L.)
Öz, Mehmet; Fidan, Muhammed Said; Baltacı, Cemalettin
In this study; aroma components, some biochemical properties (sugar amount (HPLC-DAD), vitamin C amount, water-soluble solids amount) and antioxidant capacities (bioactive component contents and antioxidant capacity amounts) of V. arctostaphylos and V. myrtillus fruits were determined. Methanol extraction was applied to the fruit samples, which were made homogeneous after being shredded. Aroma (volatile) components were determined by HS-SPME/GC-MS (Headspace-Solid Phase Micro Extraction) technique combined with Gas Chromatography-Mass Spectrometry. Total antioxidant activity (TAC), total phenolic substance amounts (TPC) and total flavonoid substance amounts (TFC), which are among the bioactive component contents, were examined. As antioxidant capacity, the amount of free radical scavenging (DPPH and ABTS) activity and the amount of ferric (III) ion reducing antioxidant power (FRAP) capacity were determined. As a result of the analyses, 27 and 33 aroma compounds were determined in V. arctostaphylos and V. myrtillus fruits, respectively. The main components found among the aroma compounds are α-terpineol (22.67%), (E)-2-hexenal (17.63%), and linalool (9.78%). in the V. arctostaphylos fruit. V. myrtillus fruits were determined to contain menthol (22.93%), α-eudesmol (11.50%), and germacrene D (8.33%). In addition, the chemical classes of aroma compounds identified were monoterpenoid, aldehyde, hydrocarbon, alcohol, ester, sesquiterpene, and sesquiterpenoid classes in V. arctostaphylos and V. myrtillus fruits. Moreover, the total sugar amount in V. arctostaphylos fruits was found to be 11.30%, and the total sugar amount in V. myrtillus fruits was 13.94%. The amount of vitamin C in V. arctostaphylos fruits was detected as 33.26 mg/kg, and the amount of vitamin C in V. myrtillus fruits was contained as 44.17 mg/kg. The amount of water-soluble solids in V. arctostaphylos and V. myrtillus fruits was determined to be 12.13% and 13.43%, respectively. The amounts of total sugar, vitamin C and water-soluble solids in V. myrtillus fruits were higher than the amounts in V. arctostaphylos fruits. According to the antioxidant results, the TAC, TPC, DPPH, ABTS and FRAP values of the methanol extract obtained from V. myrtillus fruits, excluding TFC, demonstrated higher antioxidant features than the values attained from V. arctostaphylos fruits.
- MakaleAçık Erişim2024Evaluation of Antioxidant Properties of Wild and Cultivated Blueberry Leaves and Their Phenolic Compound
Okan, Onur Tolga; Ulusoy, Esra; Öz, Mehmet; Deniz, Ilhan
Aim of study: This study aimed to investigate the antioxidant activity and polyphenol and flavonoid content of blueberry leaves, which could have positive effects on human health. Area of study: The research was conducted in three cities of Turkiye's Karadeniz region, where blueberry cultivation is prevalent: Giresun, Trabzon, and Rize. Material and method: In this study, 29 blueberry leaves (27 cultivated and two wild) were selected as sample materials. The total phenolic content (Folin-Ciocalteu method), total flavonoid content (aluminum (III) chloride method), and total antioxidant capacity (DPPH and FRAP) of the samples were analyzed. Phenolic characterization was performed using HPLC. Main results: The total phenolic content varied between 8.36 and 121.61 mg GAE/g, whereas the total flavonoid content ranged from 0.91 to 3.07 mg QE/g. The results revealed that blueberry leaves had high antioxidant activity and contained considerable amounts of polyphenols and flavonoids. Chlorogenic acid was identified as the dominant compound in all leaves samples. Research highlights: These findings indicate that blueberry leaves could be a valuable source of antioxidants in the food and cosmetic industries, comparable to the fruit itself.
- MakaleAçık Erişim2024Investigation of aroma components obtained by HS-SPME (Headspace-solid phase microextraction) method of wild strawberry (Fragaria vesca L.) and strawberry (Fragaria x ananassa Duch.) fruits; [Dağ çileği (Fragaria vesca L.) ve çilek (Fragaria x ananassa Duch.) meyvelerinin HS-SPME (Tepe boşluğu katı faz mikro ekstraksiyonu) yöntemi ile elde edilen aroma bileşenlerin araştırılması]
Öz, Mehmet; Baltaci, Cemalettin; Fidan, Muhammed Said
Wild strawberries (Fragaria vesca) are in the category of non-wood forest products due to their unique fragrance, aroma, and natural ability to grow. In this study, aroma components of wild strawberry (Fragaria vesca) and strawberry (Fragaria x ananassa) fruits were determined by Headspace-Solid Phase Micro-Extraction technique combined with Gas Chromatography-Mass Spectrometry (HS-SPME/GC-MS). In addition, it aimed to compare the results of both fruits with each other by chemical classification of the aroma components determined from wild strawberry and strawberry fruits. As a result of the HS-SPME/GC-MS analysis; 50 and 76 aroma components were determined in wild strawberry and strawberry fruits, respectively. The structure of aroma components was defined as 97.63% of wild strawberries and 98.26% of strawberries. The main components in the aroma compounds are 2-undecanone (10.38%), γ-decalactone (8.81%), and eugenol (7.14%) in wild strawberry fruit; nerolidol (29.44%), γ-decalactone (26.36%) and bisabolol oxide II (4.23%) were found in strawberry fruits. In addition, chemical classes of identified aroma compounds were determined as ester, terpene or terpenoid, fatty acids, aldehyde, and ketone classes in Fragaria vesca and Fragaria x ananassa fruits. When the results are examined, it is seen that strawberry fruits are found to be higher than wild strawberry fruits in terms of percentage of aroma components, number of aroma components, and percentage of dominant components.
- MakaleAçık Erişim2023Chemical Composition of Pistacia terebinthus L. and its Phytochemical and Biological Properties
Fidan, Muhammed Said; Baltaci, Cemalettin; Öz, Mehmet; Akar, Zeynep
Vitamin C, enzyme activities, phenolic compounds, antioxidant capacity, antimicrobial activity, and essential oil analyses of ripe and unripe fruits of P. terebinthus were investigated. Vitamin C amounts of ripe and unripe fruits were 63.2 and 15.4 mg/100g, respectively. The main phenolic compounds of unripe and ripe fruits are rutin, syringic acid, and gallic acid. It was determined that the enzyme inhibitor activities in the ripe and unripe fruits were 0.136 mg/mL and 2.14 mg/mL. In all of the free radical scavenging (DPPH and ABTS) activity, ferric (III) ion reducing antioxidant power (FRAP) capacity, total phenolic substance amounts (TPC), total flavonoid substance amounts (TFC), and total antioxidant activity (TAC) antioxidant methods analyzed with plant parts, the methanol extracts obtained from the ripe fruits of the P. terebinthus showed higher antioxidant properties than the methanol extracts obtained from the unripe fruits. Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 showed antimicrobial activity against microorganisms, while methanol extracts obtained from unripe fruit samples did not show antimicrobial activity against the microorganisms used. The chemical grade with the most compounds in the essential oils of P. terebinthus were monoterpenoids, sesquiterpenes, and monoterpenes in unripe and ripe fruits, respectively. The main components were a-pinene with 22.8% and 27.3% ratios in unripe and ripe fruits, respectively.
- MakaleYayıncıda açık erişim2023Antimicrobial, Antioxidant, and Phytochemical Activities of Rhus coriaria L. and its Phenolic Compounds and Volatile Component Analyses
Öz, Mehmet; Baltaci, Cemalettin; Fidan, Muhammed Said; Karatas, Seyda Merve
Volatile oil analysis, phenolic constituents, antioxidant capacity, antimicrobial activity, vitamin C, and enzyme activities of the fruits of Rhus coriaria L. were studied. The chemical with the highest percentage was sesquiterpene hydrocarbons with 40.4%. The major compound was detected as caryophyllene (36.9%). The main phenolic constituents of fruit samples were gallic acid, syringic acid, protocatechuic acid, and 4hydroxybenzoic acid. The highest phenolic constituent of fruits was gallic acid. Ferric (Ill) ion reducing antioxidant power (FRAP) capacity (14.9 mg FeSO4 eq./g), free radical scavenging (ABTS) capacity (68.8 mg AA eq./g), ABTS % inhibition rate (98.0%), free radical scavenging (DPPH) (53.1 mg AA eq./g), and DPPH % inhibition (79.6%) amounts were determined in antioxidant capacities of the samples. The bioactive component contents of the samples were total antioxidant amounts (TAC) (32.8 mg GA/g), total flavonoid substance amounts (TFC) (73.8 mg QE eq./g), and total phenolic substance amounts (TPC) (41.4 mg GA eq./g). The results of the antimicrobial activity analysis of R. coriaria fruit samples showed antimicrobial activity against Staphylococcus aureus and Listeria monocytogenes microorganisms. The amount of vitamin C and enzyme inhibitor activity in the fruits of R. coriaria were determined as 35.5 mg/100 g and 0.07 mg/mL, respectively.
- MakaleAçık Erişim2023Chemical composition and biological activities of essential oils from the flowers and leaves of Celtis planchoniana K.I.Chr.
Öz, Mehmet; Ucuncu, Osman; Baltacı, Cemalettin; Said Fidan, M.; Karatas, Seyda Merve
Most plant parts are used in traditional food and medicine. Plants are significant resources of potential phytochemicals. This study investigated the properties of essential oil, antioxidant, and antimicrobial activities of the flowers and leaves of the Celtis planchoniana plant. Based on the GC/MS-FID analysis of essential oils extracted from the flowers and leaves of C. planchoniana, 101 and 91 compounds were identified from their structures, respectively. Chemical classes with the highest percentage of compounds found in essential oils of plants were oil acids, with 31.51% in flowers, and aldehydes, with 32.89% in leaves. Hexadecanoic acid (29.94%) and (E)-2-hexenal (16.11%) compounds were determined as the main components in the plant’s flowers and leaves. Among the flower and leaf samples, the highest antioxidant levels were 132.77-80.10 mg AA eq./100 mL in TAC, 17.63-118.23 mg AA eq./100 mL in ABTS, 71.41-85.15 mg GA eq./100 mL in TPC, and 15.08-15.71 mg AA eq./100 mL in DPPH capacity. According to the TFC, TPC, ABTS, DPPH, and FRAP tests conducted to determine the antioxidant properties of C. planchoniana, the essential oil obtained from its leaves demonstrated more significant antioxidant properties than the essential oil obtained from its flowers. According to the antimicrobial activity analysis, the leaf and flower essential oil samples exhibited no antimicrobial activity against the microorganisms tested.
- MakaleAçık Erişim2023Chemical Content of Volatile Oil of Primula veris subsp. columnae, Obtaining the Methanol Extracts and their Biological Activities
Karapınar, Çağrı; Öz, Mehmet
The proportions and constituents of the essential oils of flowers and leaves of Primula veris subsp. columnae were determined, and the antioxidant and the antimicrobial (antibacterial and antifungal) properties of their methanol extracts were investigated. Percentage ratios and main components of the Primula veris subsp. columnae plant, which grows naturally, were detected by extracting flower and leaf volatile oils. The components of the volatile oil were identified with Gas Chromatography Mass Detector-Flame Ionization Detector (GC-MS/FID). A total of 62 compounds were identified in flower volatile oil, and methyl 4methoxysalicylate (37.1%) was determined as the main compound. While defining the structure of 50 compounds in leaf essential oil, linoleic acid (40.1%) was established as the main compound. As the result of the extraction of flowers and leaves with methanol, the extractive substance was obtained as 34.5% in flowers and as 28.8% in leaves. Methanol extraction and the antioxidant properties of the Primula veris subsp. columnae plant were quite high. In the antifungal and antibacterial activity test conducted on the volatile oils and methanol extracts of flowers and leaves of the Primula veris subsp. columnae species, only flower volatile oil showed weak inhibition properties.
- MakaleYayıncıda açık erişim2023Chemical Content and Antimicrobial Activities of Essential Oils Obtained from Plant Parts of Juniperus excelsa M. Bieb.; [Kemijski sastav i antimikrobno djelovanje eteričnih ulja dobivenih iz dijelova biljke Juniperus excelsa M. Bieb.]
Saruhan, Emrah; Öz, Mehmet
This study was conducted to determine the chemical content, antibacterial, and antifungal properties of essential oil obtained from the parts of the plant called Juniperus excelsa M. Bieb. In this study, essential oils of cone, needle, and twig of J. excelsa plant, which is a naturally grown plant in Gümüşhane province, were obtained by hydrodistillation method in a Clevenger type device. Chemical composition of essential oils was determined thanks to the analysis conducted with GC-MS/FID device. Besides, antimicrobial activity tests of essential oils were decided in contrast to 23 different microorganisms with the disc diffusion method. As a result of the essential oil analysis of J. excelsa, the percentage of essential oil yield in cones, needles, and twigs was found as 5.88 %, 2.00 %, and 0.62 %, respectively. α-pinene was confirmed to be the most abundant main compound found in the essential oils of cones, needles, and twigs. As a result of the essential oil analysis of the cone, needle, and twig of J. excelsa species, it was revealed that monoterpenes were the most abundant chemical class in terms of percentage. In the antimicrobial activity test performed on the essential oils of J. excelsa plant parts, it was found that cones, needles, and twigs have a strong antimicrobial effect against Bacillus subtilis, Enterococcus faecalis, Escherichia coli, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enteritidis, Salmonella typhimurium, Staphylococcus aureus, Candida albicans, Penicillum expansum, Saccharomyces cerevisiae bacteria, yeast, and molds.
- MakaleAçık Erişim2022Gümüşhane yöresinde yetişen Ribes petraeum Wulfen (Frenk Üzümü) bitkisinden uçucu yağının elde edilmesi, kimyasal içerik ve biyolojik aktivitelerinin belirlenmesi
Üçüncü, Osman; Ilter, Seyda Merve; Fidan, Muhammed Said; Baltaci, Cemalettin; Öz, Mehmet
Bu makalede; R. petraeum bitkisinin yapraklarının uçucu yağı Clevenger tipi cihazda su buharı-distilasyonu metodu ile alınmıştır. Uçucu yağın kimyasal bileşimleri, GC-MS/FID cihazı ile analiz edilerek belirlenmiştir. Elde edilen uçucu yağın antioksidan aktivitelerinin belirlenmesi serbest radikal giderme (DPPH ve ABTS) aktivitesine ve demir (III) iyonu indirgeyici antioksidan gücü (FRAP) kapasitesi yöntemlerine göre yapılmıştır. Ayrıca bazı biyoaktif bileşen içerikleri toplam fenolik madde miktarları (TPC), toplam flavonoid madde miktarları (TFC), toplam antioksidan aktivite (TAC) çalışmalarıyla belirlenmiştir. Uçucu yağın antimikrobiyal aktiviteleri ise disk difüzyon yöntemi ile 23 farklı mikroorganizmaya karşı belirlenmiştir. Sonuç olarak; yüzde uçucu yağ verimi R. petraeum bitkisinin yapraklarında %0.47 olarak bulunmuştur. Bitki yapraklarının uçucu yağında % oran olarak en yüksek bileşik bulunan kimyasal sınıf aldehitler (%55.98) olarak tespit edilmiştir. Ayrıca, uçucu yağında bulunan ana bileşen (E)-2-hegzenal (%52.90) olarak saptanmıştır. Bitki kısımlarının toplanan örneklerine ait elde edilen antioksidan aktivite tayininde DPPH ve ABTS miktarları sırasıyla 18.73 mg AA/100 mL ve 21.31 mg AA/100 mL aralığında ve FRAP kapasitesi 6.50 mg FeSO4/100 mL tespit edilmiştir. Biyoaktif bileşen içeriklerinin TPC miktarı 66.37 mg GA/100 mL, TFC miktarı 1.51 mg QE/100 mL ve TAC miktarı 195.43 mg AA/100 mL olduğu bulunmuştur. Bitki kısmının toplanan örneklerine ait elde edilen antimikrobiyal aktiviteleri analizleri sonucuna göre mikroorganizmalara karşı herhangi bir antimikrobiyal aktivite göstermedikleri belirlenmiştir.
- MakaleAçık Erişim2022Chemical Content and Antimicrobial Activity of Cyclotrichium origanifolium (Labill.) Manden. & Scheng. Plant Essential Oil Obtained from Adana Region
Öz, Mehmet
The aims of this paper to evaluate the chemical composition and antimicrobial properties of the essential oil obtained from the aerial parts of Cyclotricium origanifolium (Labill.) Manden. & Scheng., which grows naturally in Türkiye. The essential oil of the aerial plant parts was acquired through the hydrodistillation method in a Clevenger type device, and it was examined with GC-MS/FID. As a result of the applied hydrodistillation process, the essential oil yield was obtained as 3.63%. The structure of 66 components that were detected in the essential oil was determined by comparing with the database and literature data in the appliance. As a result of the analysis, the structure of 64 compounds was determined, but the structure of 2 compounds could not be determined. cis-3-pinanone (isocamphopinone), β-pinene and eucalyptol compounds were determined the main components respectively at the rates of 33.43%, 25.27% and 23.91%. The highest chemical compound classes in the analyzed essential oil was determined as monoterpenes with 52.84% and as monoterpenoids with 44.11%. The groups with the highest number of compounds in the chemical classes in the essential oil were monoterpenoids with 27 compounds and monoterpenes with 16 compounds. The antimicrobial activity of the essential oil was investigated with the disc diffusion test method against 13 different microorganisms activated. According to the results of the antimicrobial activities analysis of essential oil obtained from plant samples; the highest antibacterial activity was recorded against Escherichia coli O157:H7 from gram-negative bacteria with inhibition zone ranging as (4.55±0.01 mm), Staphylococcus aureus and Listeria monocytogenes from gram-positive bacteria with inhibition zone ranging as (6.50±0.01 mm), (5.65±0.01 mm) respectively. In addition, it was determined that they did not show any antifungal activity against any of the yeast- molds tested.
- MakaleYalnız künye2022Chemical Composition and Antimicrobial Properties of Essential Oils Obtained from the Resin of Picea orientalis L.
Öz, Mehmet
This study aims to evaluate the chemical composition and antimicrobial (antibacterial and antifungal) properties of the essential oil subtracted from the oleoresins, which were obtained from the stem of the Picea orientalis naturally grown in Turkey. The essential oil of the resin was obtained through the hydrodistillation method in a Clevenger type device, and it was analyzed with GC-MS/FID. The structure of 74 compounds that were found in the essential oil was elucidated by comparing with literature data and the database in the appliance. Limonene (53.40%), β-pinene (20.74%) and α-pinene (14.51%) were determined to be the major components. The highest class of compounds in resin essential oils was identified as monoterpene hydrocarbons. The antimicrobial activity of the essential oil was investigated with the agar well diffusion method against 25 microorganisms, including 13 gram-negative, 7 gram-positive bacteria and 5 yeast-molds. As the results of the analysis, the highest antibacterial activity was recorded against S. typhimurium from gram-negative bacteria with inhibition zone ranging as (13.70±0.10-9.70±0.10 mm) and S. aureus from gram-positive bacteria with inhibition zone ranging as (9.30±0.10-6.80±0.10 mm). Additionally, the highest antifungal activity was obtained against C. albicans from yeast-molds with inhibition zone ranging as (10.10±0.10-6.10±0.10 mm).
- MakaleYalnız künye2022Chemical composition, antioxidant and antimicrobial activity of Colchicum speciosum Steven growing in Türkiye
Baltacı, Cemalettin; Öz, Mehmet; Fidan, Muhammed Said; Üçüncü, Osman; Karataş, Şeyda Merve
In the current study, the chemical constituents, antioxidant and antimicrobial activity of the volatile oil obtained from flowers and stems of C. speciosum growing in Türkiye were investigated. Both volatile oils were extracted through the hydrodistillation method in a Clevenger apparatus followed by the GC-MS analysis. The volatile oil yield attained from flowers and stems parts were determined as 0.62% and 0.65%, respectively. The most abundant compounds were determined in the flowers. Tricosane, octadecanal and hexadecanoic acid were identified to be the major components. Tricosane (38.28%) was defined to be the major component in flowers. 2-hydroxy benzaldehyde, tricosane and hexadecanoic acid were found to be the main compounds in the volatile oil of stems. 2-hydroxy benzaldehyde (46.49%) was found to be the main constituents in stems. According to the results antioxidant activity was attained as total antioxidant substance amount (86.77 mg AA/100 mL), total phenolic substance amount (65.64 mg GA/100 mL), total flavonoid substance amount (3.92 mg QE/100 mL), ferric (III) ion reducing antioxidant power capacity (6.88 mg FeSO4/100 mL) and free radical scavenging capacity (ABTS) (50.67 mg AA/100 mL) were obtained the highest antioxidant properties were obtained in flowers of C. speciosum. C. speciosum volatile oils had antimicrobial activity against Bacillus cereus in the flowers and had no activity against bacteria and yeast-mould in the stems.
- MakaleYalnız künye2021Determination of the Chemical Composition, Antioxidant and Antimicrobial Activities of Different Parts of Rosa canina L. and Rosa pimpinellifolia L. Essential Oils
Öz, Mehmet; Deniz, Ilhan; Okan, Onur Tolga; Baltaci, Cemalettin; Karatas, Seyda Merve
Today, the use of essential oils is increasing day by day, and it is considered as raw materials in many industries. Therefore, this study aims to identify the essential oil profile, antioxidant and antimicrobial activity of Rosa canina L. and Rosa pimpinellifolia L. reveal their potential use in the industry. Essential oils that were obtained in 2013-2014 from different parts (flower, leaf, stem, and fruit) of Rosa canina L. and Rosa pimpinellifolia L. by the hydro-distillation method were analyzed by GC/MS. The dominant components in Rosa canina L. were found as trans-geraniol in the flowers (42.08 % for 2013), linalool in the leaves (11.55 % for 2014), and stem (14.91 % for 2013) and vitispirane in the fruits (37.19 % for 2014). The dominant components in R. pimpinellifolia L. were found as heneicosane in the flowers (22.07 % for 2014), γ-elemene in the leaves (30.21 % for 2013), linalool in the stem (14.76 % for 2013) and the fruits, (E)-2-hexenal for those collected in 2013 (14.09 %). The IC50 and FRAP values of the samples ranged from 127.54 to 142.22 μg/g and 121.43 to 296.78 μmol TE/g, respectively. According to the results obtained, both R. canina L. and R. pimpinellifolia L. were found to show poor antioxidant values of DPPH and FRAP. In general, it was determined that the essential oils obtained from different plant parts of both species had a moderate antimicrobial effect against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, and Pseudomonas aeruginosa.
- MakaleAçık Erişim2021Determination of Antimicrobial and Antioxidant Activities and Chemical Components of Volatile Oils of Atropa belladonna L. Growing in Turkey
Öz, Mehmet; Fidan, Muhammed Said; Baltaci, Cemalettin; Ücüncü, Osman; Karatas, Seyda Merve
In this study; the essential oils were obtained from the flowers and leaves of Atropa belladonna L. plants through the hydrodistillation method in a Clevenger type device. The chemical composition of volatile oils was determined by analyzing with the GC-MS/MS instrument. The antioxidant activity of the obtained volatile oils was determined according to total antioxidant content (TAC), free radical scavenging (ABTS) capacity, free radical scavenging (DPPH) amounts, total flavonoid content (TFC), total phenolic content (TPC), and ferric reducing antioxidant power (FRAP) capacity methods. Antimicrobial activities of essential oils were determined through the agar-disc diffusion method. As for the results; volatile oil yields of the A. belladonna were determined as 0.37 % in leaves and 1.57 % in flowers. The highest percentage of chemical compounds in essential oils of the A. belladonna plant were determined as (43.75 %) aldehydes in leaves and as (39.96 %) fatty acids in flowers. In addition, the main constituents found in volatile oils were eicosane (35.92 %) in the leaves and hexadecenoic acid (18.84 %) in the flowers of the A. belladonna. The highest amounts of antioxidant for the test specimens were found as 1.37-1.48 mg QE/100 mL in TFC, as 13.25-21.91 mg AA/100 mL in ABTS, as 51.49-62.87 mg GA/100 mL in TPC, and as 4.71-7.97 mg FeSO4/100 mL in FRAP capacity. As the results of the antimicrobial activities, it was determined that only the leaves of A. belladonna show antimicrobial activity against the mold-yeast and gram-positive and gram-negative bacteria microorganisms among the 17 bacteria and 6 yeast-mold types used in the study.
- MakaleYalnız künye2020Determination of chemical composition, antioxidant and antifungal properties of pomegranate(Punica granatum L.) and Parsley (Petroselinum crispum) seed oil produced in industrial scale
Okan, Onur Tolga; İlhan, Hatice Deniz; Onaran, Abdurrahman; Kılıc Pekgözlü, Ayben; Öz, Mehmet
In this study, essential oil and oil acid content, antioxidant and antifungal properties of oils obtained from pomegranate (Punica granatum L.) and parsley seeds (Petroselinium crispum) produced on an industrial scale were investigated. Pomegranate seed oil was obtained cold pressed in an industrial scale, while parsley seed oil was obtained industrial steam distillation. As a result of pomegranate seed oil GC-MS analysis, fifteen components were determined. Parsley seed oil was twelve compounds was identified. Punicic acid (61.19 %) was found as the dominant compound in pomegranate seed oil while apiole (14.21 %) was determined as the dominant compound in parsley seed oil. When the antioxidant capacity of the oils were examined, it was determined that the oils obtained from pomegranate seeds have a moderate antioxidant activity, the oils obtained from parsley seeds have high antioxidant activity. Antifungal activity of pomegranate (Punica granatum L.) and parsley (Petroselinium crispum) seed oil against five different plant pathogens, Fusarium. oxysporum f. sp. lycopersici, Botrytis cinerea, Sclerotinia sclerotiorum, Alternaria. solani andRhizoctonia solani were also determined.
- MakaleYalnız künye2019Recreational Use of Protected Areas Found in The Eastern Black Sea Region
Fidan, Muhammed Said; Komut, Osman; Öz, Mehmet; Adanur, Hakan
Protected areas are areas where educational and recreational use of natural and cultural areas with special precaution is provided where educational activities and scientific research are carried out, genetic diversity and species are kept, cultural, traditional, symbolic natural resources are avoided and sustainable from damaging effects. Recreation is, as a definition, expressed as activities that people make with their own will in their free time in order to restore old weariness of soul and body health. In this study, the protected areas in the provinces of Artvin, Giresun, Gümüşhane, Rize and Trabzon in Eastern Black Sea Region were selected as material. It is aimed to determine and compare visitor numbers between 2010-2017 in these areas. The visitor numbers of provinces were compared using one way ANOVA. As of 2018, there are 3 protected areas in Artvin, 5 in Gümüşhane, 8 in Trabzon, 6 in Rize and 4 in Giresun as recreational use. Total protected areas within the boundaries of the regional directorate are 450,411 in 2010, 555,678 in 2011, 973,391 in 2012, 1,936,608 in 2013, 1,601,913 in 2014, 1,458,617 in 2015, 1,558,290 in 2016, and finally 1,911,429 in 2017 people visited the site. As a result, visitor numbers have increased in 2010, 2011, 2012 and 2013 and there has been no decrease in 2014 and 2015. In 2016 and 2017, the number of visitors has increased again. The largest number of visitors was registered in 2013, and the least number of visitors was registered in 2010. The most visited province was Trabzon while the least visited was in Giresun province. It can be said that as the number of visitors increases with the number of years, it is important that the number of declared protected areas increase, the protected areas become more capable of providing quality services, and the recreation needs of people increase gradually.