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Yayınlar
- MakaleAçık Erişim2020The Ameliorative Effects of Ethyl Pyruvate and Dimethyl Sulfoxide on Ischemic Tissue Injury in Experimental Carbon Monoxide Intoxication
Pasli, Sinan; Imamoglu, Melih; Çiçek, Mustafa; Yadigaroglu, Metin; Sahin, Aynur; Karaca, Yunus; Yulug, Esin; Tatli, Ozgur
Aim: The purpose of this study was to investigate the protective effects of ethyl pyruvate (EP) and dimethyl sulfoxide (DMSO) on ischemic tissue in brain and cardiac and hepatic tissues in experimentally induced carbon monoxide (CO) intoxication. Material and Methods: Thirty-five mature female Sprague Dawley rats were randomized into five groups of seven animals each. Group 1 received no CO or treatment. Rats in groups 2, 3, 4 and 5 were made to inhale a high-concentration 5000 ppm CO gas mixture for 60 min at 4 L/min. CO levels were then measured from 1 mL tail vein blood. Group 2 received no therapeutic agent, group 3 received 6 mg/kg intraperitoneal (ip) DMSO, group 4 received 50 mg/kg ip EP, group 5 received 50 mg/kg EP and 6 mg/kg DMSO ip. All rats were sacrificed by decapitation. Brain, cardiac and hepatic tissues were removed and histopathological scores were compared. Results: Comparison of group 2 with group 3 and group 2 with group 5 revealed that DMSO alone and EP + DMSO exhibited a reducing effect on degree of cerebral neuronal alteration, degenerative neuron rates, and total cardiac injury score (p=0.005, p=0.002, p=0.004 and p=0.002, p=0.001, p=0.004 respectively). There was no histopathological difference between group 2 and group 4 suggesting EP alone had no therapeutic effect on histopathological injury. Conclusion: Based on our study findings, EP administered alone exhibited no protective effects on the organ injuries investigated, while DMSO exhibited reducing effects on degree of neuron alteration, rates of degenerative neurons, and total cardiac injury scores. An ameliorating effect on cardiac and hepatic injury was more prominent with combined treatment.
- MakaleAçık Erişim2020The Value of Cardiotrophin-1 in the Diagnosis, Severity, and Prognosis of Pulmonary Embolism
Pasli, Sinan; Asik, Olgun; Imamoglu, Melih; Çiçek, Mustafa; Yadigaroglu, Metin; Dogramaci, Seniz; Mentese, Ahmet; Sahin, Mursel; Tatli, Ozgur
Aim: Pulmonary embolism (PE) is a life-threatening condition that requires early diagnosis and treatment. There is still a need for precise and non-invasive biomarkers that can be used to diagnose PE. This study aimed to determine the diagnostic value of Cardiotrophin-1 (CT-1) in patients with acute PE and ascertain the relationship between serum CT-1 level and disease prognosis and severity. Materials and Methods: In this prospective study, the serum CT-1 levels of 165 patients with suspected acute PE were measured. The patients were divided into two groups, PE (+) and PE (-), based on their computed tomography angiography results. Their serum CT-1 levels were compared. Results: The CT-1 levels of the PE (+) group (median=4.18 pg/mL, range=3.57-9.55 pg/mL) was non-significantly (p=0.08) different from those of the PE (-) group (median=4.38 pg/mL, range=3.57-51.64 pg/mL). Based on the severity of the condition, there were significant differences (p=0.001) in the CT-1 levels of the patients in the intermediate-low risk group (median=4.19 pg/mL, range=3.62-5.32 pg/mL), intermediate-high risk group (median=4.06 pg/mL, range=3.57-9.31 pg/mL) and high risk group (median=5.01 pg/mL, range=3.89-9.55 pg/mL). Based on the clinical course, the CT-1 levels of the patients in the good clinical course group (median=4.15 pg/mL, range=3.57-9.31 pg/ml) were significantly different (p=0.004) from those in the poor clinical course group (median=4.53 pg/mL, range=3.66-9.55 pg/mL). Conclusion: CT-1 level was not observed to be a precise biomarker for the diagnosis of acute PE. However, our study demonstrated that the serum CT-1 levels can be used as a biomarker for ascertaining the severity and prognosis of patients with PE.