In vitro Multiplication of Black Mulberry (Morus nigra L.)

In this study, which was aimed to determine the multiplication potentially of black mulberry (Morus nigra L.) by tissue culture, in order to standardize an effective protocol oriented to provide multiple shoots of nodal explant obtained from glutton branch of adult tree, the MS mediums, which were enriched with different concentrations and combinations of plant growing regulators, were tried. The MS mediums, at 5 different concentrations of BAP (6-benzylaminopurine; 1.0, 2.0, 3.0, 4.0, 5.0 mg/L) and also a combination of BAP, NAA (naphthalen acetic acid; 0.25 mg/L) and TDZ (Thidiazuron; 0.1 mg/L), were used. After 45 days of inoculation, the regeneration percentage of shoots, the numbers of shoots per explant (shoots/explant), average shoots length (cm), the number leaf per explant (leaf/explant) were determined. The MS medium, supported with BAP (2.0 mg/L), TDZ (0.1 mg/L) and NAA (0.25 mg/L), was the most effective with respect to trunk regeneration and all other parameters. The shoots obtained from in vitro were taken to rooting mediums. In rooting mediums the best rooting percentage and the number of roots per explant were obtained with a combination usage of BAP (1.0 mg/L) and NAA (0.5 mg/L) The data obtained from this study presents a protocol for the serial productions mulberry with tissue culture. On the other hand the results of this study will provide a basis for further studies