One-step kinetic analysis of Listeria innocua growth as a surrogate for Listeria monocytogenes on arugula leaves with background microbiota: model development and validation

This study investigated the growth kinetics of L. monocytogenes and L. innocua on fresh arugula leaves under abusive conditions, considering the presence of background microbiota (BM) including (APC (Aerobic Plate Count), TPC (Total Psychrotrophic Count), and LAB (Lactic Acid Bacteria)). Additionally, the feasibility of using L. innocua as a surrogate for L. monocytogenes was evaluated for industrial practices. Predictive models were developed to assess the effect of temperature and time on the growth kinetics of both microorganisms. Experiments were conducted in triplicate to observe growth kinetics at temperatures from 5 to 35°C. The growth curves were analyzed using one-step analysis with the USDA IPMP-Global Fit software, employing the Huang full/no-lag phase growth as the primary models and the Huang sub-optimal (HSRM) and Ratkowsky sub-optimal square-root as the secondary models. An additional set of isothermal data, collected at 15°C and 20°C, was used to validate the models. Results showed that the minimum growth temperatures were 2.91±0.50°C for L. monocytogenes and 2.88±0.50°C for L. innocua, while 2.05±0.89, 1.93±0.98 and 3.55±1.97°C for APC, TPC and LAB, respectively. The specific growth rates of L. monocytogenes and L. innocua ranged from 0.01 to 0.93 h⁻¹. The root mean square error (RMSE) of model validation and development was less than 0.3 log CFU/g, indicating that the combination of the Huang growth model with HSRM could accurately predict the growth of L. monocytogenes under abusive conditions. Validated models can provide useful input to quantitative risk assessment tools to predict the growth of L. monocytogenes on arugula leaves in the presence of BM during distribution or storage. The findings of this study support the use of L. innocua with R2=0.961 as a suitable surrogate in industrial practices for fresh produce.