Sürdürülebilir Kalkınma Amaçları

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• MakaleAçık Erişim2022/r/gumushane/20442
  Effect of Brewing Conditions on Sensorial and Antioxidant Properties of Sage Tea

  Ilyasoglu, Huri; Zemzemoglu, Tuba Eda Arpa

  In this study, the effects of infusion time and temperature on the sensorial properties and antioxidant capacity of sage tea were evaluated by using the response surface methodology. The obtained quadratic models explained more than 90% variability in the responses. The infusion temperature showed significant negative effect on the sensorial properties whereas it had significant positive effect on the total phenolic content and antioxidant capacity (p<0.05). Moreover, the infusion time showed significant negative effects (p<0.05) on the responses. The best combination of brewing conditions was determined as 75-80 degrees C and 2-4 min.

  AtıfWoS1·OpenAlex1

  İncele
• MakaleAçık Erişim2021/r/gumushane/16640
  Investigation the Different Extraction Temperature Effect of Artvin Savsat Region Propolis PBS Extracts on Antioxidant Properties and Eryhrocyte Hemolysis Inhibition

  Canbolat, Deniz; Turan, Ibrahim; Küpeli, Yunus Emre; Kilinç, Sedanur; Piliç, Sevim

  Propolis, which has been used in traditional medicine for hundreds of years all over the world, is a resinous natural product with a complex content obtained by honey bees by mixing the buds of plants and pollen grains with enzymes they secrete. Propolis may have different contents due to the gathering region, plant source, bee variety and climate of the place where it is collected. Although there are many studies related to different regions, so far the propolis of the Savsat region in Artvin have not been studied yet. The aim of this study is to determine the antioxidant properties (determination of iron reducing power, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, total phenolic and total flavonoid content) of propolis extracts of Artvin Savsat region prepared with phosphate buffer salt solution (PBS) prepared at different temperatures (45 and 60 degrees C) and to determine the erythrocyte hemolysis inhibition properties of the extracts. Total phenolic (11.91 +/- 0.5-24.14 +/- 1.38 mg gallic acid equivalent to g propolis) and total flavonoid (1.79 +/- 0.03-2.13 +/- 0.07 mg quercetin equivalent to g propolis) content of PBS extracts, iron power determination (12.80 +/- 0.36-27.15 +/- 0.12 mg ascorbic acid equivalent to g propolis), 2,2-diphenyl-1-picrilhydrazyl (DPPH) radical scavenging activity (%22.86 +/- 0.5-31.24 +/- 1.33) and erythrocyte hemolysis inhibition (%55.58 +/- 0.57-96.37 +/- 0.02) were determined using spectrophotometric methods. As a result of the experiments, it was determined that the extract with PBS obtained at a temperature of 60 degrees C was higher than the extract obtained at 45 degrees C, in terms of antioxidant properties and erythrocyte hemolysis inhibition ratio. Further studies are required to identify molecules that inhibit erythrocyte hemolysis in extracts and have antioxidant properties.

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  AtıfWoS4·OpenAlex9·TR Dizin5

  İncele
• MakaleAçık Erişim2021/r/gumushane/16410
  Variation of Bioactivities and Phytochemical Compositions of Valeriana dioscoridis Sm. Extracts

  Uçar, Esra; Atas, Mehmet; Çilesiz, Yeter; Çinbilgel, Ilker; Eruygur, Nuraniye; Karaköy, Tolga

  Valerian (Valeriana dioscoridis Sm.) is a perennial herb of the Caprifoliaceae family. The aim of this study was to determine the variation of V. dioscoridis plants grown in different regions in respect of the quality criteria traits such as nutrient values, extract contents and in vitro antioxidant and antimicrobial activity values. In this study, roots and rhizomes of V. dioscoridis Sm. were collected from eight different locations in Antalya province. The obtained materials were ground in a blender and extracted with 80% ethanol. The macro and micro-nutrient elements contents of the powdered plant samples were evaluated and the antioxidant and antimicrobial activities of the extracts were investigated. Based on the data obtained, there were different values in the antioxidant, antimicrobial activities, nutrient content and chemical composition content of extracts of V. dioscoridis Sm. plants grown in different locations.

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  AtıfOpenAlex1

  İncele
• MakaleAçık Erişim2021/r/gumushane/16314
  The Effect of Rosa pimpinellifolia Extract on the Proliferation of Human Tumor Cells

  Demir, Elif Ayazoglu; Demir, Selim; Türkmen, Nihal; Turan, Ibrahim

  The aim of this study was to evaluate the antioxidant properties of R. pimpinellifolia and its cytotoxic effect against human colon (WiDr), liver (HepG2) and lung (A549) cancer cells compared with a normal fibroblast (BJ) cell line. The antioxidant properties of extract was determined using spectrophotometric methods, while the cytotoxic effect of extract was evaluated using MTT assay. The total phenolic content, total flavonoid content and reducing power values of extract were calculated as 16.4 +/- 0.4 mg gallic acid equivalent, 5.2 +/- 0.2 mg quercetin equivalent and 34.3 +/- 2.4 mg trolox equivalent per g sample, respectively. Although the extract exhibits selective cytotoxic effect on three cancer cell lines in a dose-dependent manner compared to BJ cells, the highest selectivity index value (2.7-fold) was obtained for the WiDr cell line. In conclusion, this is the first study to demonstrate the cytotoxic effect of R. pimpinellifolia on cancer cells. To demonstrate the molecular mechanism of this cytotoxic effect and to carry out in vivo studies may be a guide for future clinical studies.

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  AtıfWoS3·OpenAlex5·TR Dizin1

  İncele
• MakaleAçık Erişim2021/r/gumushane/16277
  Evaluation of Cytotoxic Effect of Onosma armeniacum Extract on Various Cancer Cells

  Demir, Selim; Ayazoglu Demir, Elif; Turan, Ibrahim; Özgen, Ufuk

  The aim of this study was to investigate the antioxidant properties of the root extract of Onosma armeniacum and its cytotoxic effect against human liver (HepG2), lung (A549) and colon (WiDr) cancer cell lines compared with a normal fibroblast cell line. Antioxidant properties of the extract was determined using the colorimetric methods. The cytotoxic effect of the extract were investigated using MTT assay. The total phenolic content and reducing power values of extract were 26.1 +/- 0.4 mg gallic acid equivalent and 52.6 +/- 1.1 mg trolox equivalent per g sample, respectively. The extract exhibited selective cytotoxic effect in all studied cancer cell lines, especially HepG2 (3.6-fold). The results of this study reveal that O. armeniacum, which has been found to have acceptable antioxidant and cytotoxic effects, should be examined with more comprehensive studies.

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  AtıfWoS8·OpenAlex12·TR Dizin1

  İncele
• MakaleAçık Erişim2021/r/gumushane/15861
  Investigation of the Cytotoxic Effect of Ethyl Pyruvate on Various Cancer Cell Lines

  Ayazoglu Demir, Elif; Demir, Selim; Turan, Ibrahim

  Ethyl pyruvate (EP) is a simple aliphatic ester derived from pyruvic acid which is an endogenous metabolite. Although various studies have investigated the antioxidant and anti-inflammatory properties of EP, there has been only limited research into the cytotoxic effect of EP on cancer cells. The aim of this study was to determine the cytotoxic effects of EP on cells representing common cancer types. EP was purchased commercially and intermediate stock solutions were prepared with phosphate buffer saline. The cytotoxic effect of EP on human melanoma (VMM917), cervix (HeLa), breast (MCF-7), lung (A549), liver (HepG2), colon (WiDr) cancer and normal fibroblast (BJ) cells was determined using the MTT assay. Cisplatin was used as a positive control in cytotoxicity experiments. The results showed that EP exhibits selective cytotoxic effect on VMM917 (10.1-fold) and HeLa (3.04-fold) cells compared to BJ cells. This study shows for the first time that EP has a highly selective cytotoxic effect, especially on melanoma and cervix cancer cells. The mechanism of this effect needs to be elucidated by more extensive studies.

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  AtıfWoS2·OpenAlex2

  İncele
• MakaleAçık Erişim2019/r/gumushane/16384
  Selective Cytotoxic Effect of Dorycnium pentaphyllum Extract on Human Breast, Liver, and Lung Cancer Cells

  Demir, Selim; Turan, Ibrahim; Misir, Sema; Aliyazicioglu, Yuksel

  Although some studies have evaluated the cytotoxic activity of different Fabaceae species, there has been only limited research into the cytotoxic effect of Dorycnium pentaphyllum. The purpose of this study was to determine the antioxidant properties and cytotoxic effect of D. pentaphyllum extract on various human cancer cells. The total phenolic content (TPC) of the extract was determined using colorimetric method. The cytotoxic effect of the extract on human lung (A549), liver (HepG2), and breast (MCF-7) cancer cells and a normal human fibroblast cells was assessed using the MTT assay. TPC value of extract was found as 140.3 +/- 1.1 mg gallic acid equivalent per g sample. Extract showed selective cytotoxicity on all studied cancer cells compared to normal fibroblast cells, and the IC50 values of the extract in the cancer cells range from 100.4 to 298.5 mu g/mL. This is the first study to reveal the cytotoxic effect of D. pentaphyllum extract on cancer cell lines. Phytomedical applications of D. pentaphyllum may represent promising approaches in the treatment of cancer.

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  AtıfWoS7·OpenAlex7·TR Dizin5

  İncele
• MakaleAçık Erişim2019/r/gumushane/16365
  Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells

  Aliyazicioglu, Yuksel; Demir, Selim; Yaman, Scrap Ozer; Sener, Sila Ozlem; Demir, Elif Ayazoglu; Aliyazicioglu, Rezzan; Turan, Ibrahim

  The purpose of this study was to investigate the phytochemical analysis of D. pentaphyllum and its cytotoxic activity on human cervical cancer (HeLa) cells with possible mechanisms. Total phenolic contents (TPC) and phytochemical analysis of the extract were evaluated using spectrophotometric method and RP-HPLC, respectively. The cytotoxic effect of the extract was evaluated using the MTT assay. The mechanism of the cytotoxic effect of the extract was also evaluated in terms of apoptosis, cell cycle, and mitochondrial membrane potential (MMP) using the fluorometric methods. The TPC of the extract was calculated as 68.9 +/- 2.3 mg gallic acid equivalent per g sample and quercetin was determined as the major phenolic in the extract. The extract showed a selective cytotoxic effect (6.5-fold) on HeLa cells compared to normal fibroblast cells. The cytotoxic effect of the extract was found to be due to the arresting of the cell cycle in the S phase and increasing apoptosis through decreased MMP. Further studies are needed for the comprehensive effects of the extract on cell signaling.

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  AtıfWoS11·OpenAlex11·TR Dizin7

  İncele
• MakaleAçık Erişim2019/r/gumushane/16261
  Antioxidant Properties of Primula vulgaris Flower Extract and Its Cytotoxic Effect on Human Cancer Cell Lines

  Demir, Selim; Turan, Ibrahim; Aliyazicioglu, Yuksel

  Primula vulgaris is a plant belonging to Primula genus, and these species are often used in traditional therapy. Although there are numerous studies on the antioxidant and cytotoxic effects of different Primula species, there are limited studies of these properties of P. vulgaris extracts. The aim of this study was to determine the antioxidant properties and cytotoxic effects of the extract prepared with dimethyl sulfoxide from P. vulgaris flowers. The total phenolic content, total flavonoid content, and reducing antioxidant power of the extract were determined using spectrophotometric methods. The cytotoxic effect of the extract on human colon (WiDr), lung (A549), liver (HepG2), breast (MCF-7), and prostate (PC-3) cancer cells and a normal human fibroblast cell line was assessed using the MTT assay. Total phenolic content and reducing power values of extract were found 33.02 +/- 0.92 mg gallic acid equivalents, and 64.86 +/- 1.18 mg trolox equivalents per g sample, respectively. Extract exhibited selective cytotoxic effect on all studied cancer cells compared to normal fibroblast cells, and the IC50 values of the extract in the cancer cell lines range from 191.8 to 375.3 mu g/mL. This work is one of the pioneering work that reveals the powerful antioxidant properties of P. vulgaris flower extract and its selective cytotoxic effect on cancer cell lines. Further studies are needed to identify the active molecules in the extract and the cytotoxic action mechanisms of these molecules.

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  AtıfWoS20·OpenAlex22·TR Dizin9

  İncele
• MakaleAçık Erişim2018/r/gumushane/16262
  Cytotoxic Effect of Rhododendron luteum Leaf Extract on Human Cancer Cell Lines

  Demir, Selim; Turan, Ibrahim; Aliyazicioglu, Yuksel

  Rhododendron luteum belongs to the genus Rhododendron, members of which are frequently used in folk medicine. Various studies have investigated the cytotoxic effect of different Rhododendron species, but there have been limited studies of the cytotoxic effect of R. luteum. The aim of this study was to investigate the antioxidant properties and cytotoxic effect of R. luteum leaf extract on the human cancer cells. The total phenolic content (TPC), total flavonoid content (TFC), and reducing power of the extract were evaluated using spectrophotometric procedures. The cytotoxic effect of the extract on five cancers (human breast, colon, lung, prostate, and liver carcinoma) and human fibroblast cells was determined using the MTT assay. TPC and reducing power values of extract were found 173.2 +/- 3.97 mg gallic acid equivalents, and 629.5 +/- 3.49 mg trolox equivalents per g sample, respectively. R. luteum leaf extract exhibited selective cytotoxicity especially against colon (1.9 fold) and liver (2.2 fold) cancer cells compared to normal fibroblast cells. This is the first study to reveal the cytotoxic effect of R. luteum leaf extract. Further studies are now needed to identify the cytotoxic molecules in the extract and their mechanisms.

  AtıfWoS15

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