Paksoy, Zahid

In this study, the effects of 4-Vinylcyclohexene diepoxide (VCD) on the testicular gene expression in dogs were investigated. Eighteen male dogs were used as material and were randomly allocated to 3 groups. The first group (n=6) was administered sesame oil by intraperitoneal (i.p.) route once a day for 8 days, and this group was served as a control. The second group (n=6) received 80 mg/kg VCD and the third (n=6) group was administered VCD (i.p.) at a dose of 320 mg/kg once daily for 8 days. The testes were removed on following day the last VCD administration. The testes were immediately placed in the RNA stabilization solution and stored at -20°C. Later mRNAs were obtained from tissue samples. After RNA isolation, the cDNAs were synthesized. Finally, MasterMix was prepared and gene expression levels were examined at the real-time PCR. The expression of B cell lymphoma 2 (Bcl-2), tumor suppressor protein (tp53), Bax, CASP2, CASP3, CASP8, CASP9 and β-actin were investigated in the all groups. Cross-group comparisons were made by using student t test. The tp53 gene expression in the second group was found to be higher compared to the control group. It was determined that the expression of Bcl-2 was reduced in the testes of animals treated with high-dose VCD. CASP-3 was found to be more expressed and CASP-9 was down-regulated in the third group. In addition, CASP-8 gene expression was significantly reduced in both treatment groups. As a result, it can be concluded that expression changes in the tp53, Bcl-2, CASP-3, CASP-8 and CASP-9 genes in the testes of male dogs may cause apoptotic changes with the VCD application.

Paksoy, Zahid

In this study, it was aimed to make the pregnancy diagnosis with the determination of glycoproteins (PAG) associated pregnancy after insemination in the cows, and to determine the late embryonic mortality rates. As material, 2919 head cows in different races and ages were used in farms and family businesses in Kahramanmaraş province. Milk sample was taken on the 25–30 day after artificial insemination from cows. The level of PAG in these milk samples was analyzed by using the Milk Pregnancy Test Kit for cows. This kit was used according to the manufacturer's instructions. During the study, it was determined that 1660 cows were pregnant, 188 were suspicious and 1071 were not pregnant. The pregnant animals were examined by rectal palpation on the 50th day when their pregnancy was continued. Controls performed on the 50th day of pregnant cows showed that 1623 cows were still pregnant and 37 cows had late embryonic deaths. As a result, early pregnancies can be accurately detected by measuring the level of PAG in the cow's milk using the Milk Pregnancy Test Kit. It was concluded that late embryonic mortality rates could be detected and that this analysis could be used routinely.