Sürdürülebilir Kalkınma Amaçları

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• MakaleYalnız künye2021/r/gumushane/14625
  N epeta meyeri essential oil ameliorates fungal infection and the antioxidant response in grapevines (Vitis vinifera) infections by gray mold (Botrytis cinerea)

  Karakuş, Sinem; Atici, Ökkeş; Köse, Cafer; Aydin, İhsan

  The essential oil (EO) obtained from Nepeta meyeri Benth. used to struggle allelopathically with gray mold (Botrytis cinerea Pers.), which causes significant damage to vineyards (Vitis vinifera L. cv. Karaerik). The EO from N. meyeri was determined to contain 4aα, 7α, 7aα-nepetalactone (71.68%) at the highest rate when analyzed with GC/MS. Then, the doses (0.6, 0.8, and 1.0 µL/leaf) of 1 ppm EO solution were applied to the leaves together with the fungus inoculation (EO + F), as well as 1 day (EO + F1D) and 2 days (EO + F2D) before inoculation. The fungus application alone was evaluated as a control. Seven days after the inoculations, fungus development, lipid peroxidation level (as malondialdehyde), the contents of reactive oxygen species (ROS), photosynthetic pigments and soluble proteins, and the activities of antioxidant enzymes (superoxide dismutase, catalase, peroxidase, and glutathione reductase) were determined in the leaves. Findings revealed that EO + F1D among the applications and 1.0 µL/leaf among the EO doses significantly prevented fungus development on the leaf, compared to the control. Moreover, the same applications decreased ROS levels, such as H2O2 and O2·− and lipid peroxidation, while they increased antioxidant enzyme activities, as well as chlorophyll and soluble protein contents. Therefore, it can be suggested that the EO from N. meyeri has a potentially powerful usage as biofungicide in the allelopathic struggle against the infection of B. cinerea in vineyards.

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• MakaleYalnız künye2019/r/gumushane/15305
  Psychrotolerant bacteria isolated from the leaf apoplast of cold-adapted wild plants improve the cold resistance of bean (Phaseolus vulgaris L.) under low temperature

  Tiryaki, Deniz; Aydın, İhsan; Atıcı, Ökkeş

  We have isolated psychrotolerant bacteria from the leaf apoplast of cold-adapted wild plants and aimed to investigate their effect on the cold resistance of bean (Phaseolus vulgaris L.). Based on the findings of 16S rRNA gene sequence analysis, 20 isolates belonging to 5 bacteria species (Pseudomonas fragi, P. chloropaphis, P. fluorescens, P. proteolytica and Brevibacterium frigoritolerans) were identified in the leaf apoplastic fluid of Draba nemorosa, Galanthus gracilis, Colchicum speciousum, Scilla siberica, Erodium cicutarium, respectively. We have determined that 6 of the 20 isolates have exhibited ACC (1-aminocyclopropane-1-carboxylate) deaminase activity and secreted different extracellular proteins under cold condition (+4 °C) compared to normal growth condition (28 °C). The six isolates were then inoculated independently of each other to the leaves of 10-day-old bean seedlings growing under normal conditions (25/22 °C, 16/8 h photoperiod), and the inoculated and uninoculated (control) seedlings were transferred to cold (9/5 °C, 16/8 h photoperiod) for 3 days. The bacterial inoculations have decreased freezing injury, ice nucleating activity and lipid peroxidation content in parallel with the decrease of reactive oxygen species level such as O2.- and H2O2 in the inoculated seedlings compared to the control. In addition, the inoculations of the isolates have stimulated the activity of apoplastic antioxidant enzymes including superoxide dismutase, catalase, peroxidase, and glutathione reductase. The results show that the inoculations improve the cold resistance of bean seedlings and the psychrotolerant bacterial isolates can be evaluated within the group of plant growth promoting bacteria (PGPB) which can increase tolerance of cold-sensitive crops.

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