Sürdürülebilir Kalkınma Amaçları

• 2

• MakaleAçık Erişim2025/r/gumushane/14187
  The Correlation Between Circulating Fetuin-A Levels and Fetuin-A to Adiponectin Ratio With Obesity in a Turkish Population: A Case-Control Study

  Icer, Mehmet Arif; Koçak, Tevfik; Ağagündüz, Duygu; Yıldıran, Hilal

  The relationship between serum fetuin-A levels and obesity has not been fully understood. This study aims to elucidate the levels of serum fetuin-A and F/A ratio in obesity and contribute to the literature on whether they could be novel clinical predictors modulated through body composition and nutrition. The study included 73 participants who were obese and 71 participants with normal weight, aged between 18 and 65 years. Certain anthropometric measurements of the individuals were taken, and a body composition analysis was performed. In addition, 3-day food records (consecutive days: 2 weekdays and one weekend day) were taken. Serum levels of fetuin-A (ng/mL) and adiponectin (μg/mL) were measured using an enzyme-linked immunosorbent assay (ELISA) method. The F/A ratio was obtained by dividing fetuin-A (ng/mL) by adiponectin (μg/mL). Serum fetuin-A (ng/mL) level and F/A ratio are higher in obese participants than in normal weight (p < 0.05). Body weight (kg) (r = 0.553, p ≤ 0.001), body mass index (kg/m2) (r = 0.492, p ≤ 0.001), waist circumference (cm) (r = 0.522, p ≤ 0.001), body fat percentage (%) (r = 0.203, p = 0.015), serum fasting blood glucose levels (mg/dL) (r = 0.317, p ≤ 0.001), and dietary protein intake (kg) (r = 0.165, p = 0.048) were observed to be positively correlated with serum fetuin-A levels. Positive correlations were also observed between these parameters and the F/A ratio (p < 0.05). Negative correlations were found between serum adiponectin and these parameters (p < 0.05). In addition, when dietary cholesterol intake was kept constant, a 1-unit increase in dietary energy intake (kcal) resulted in a 0.043-fold increase in serum fetuin-A (p < 0.05). The study results support theories suggesting the involvement of serum fetuin-A levels and the F/A ratio in the pathogenesis of obesity. The higher serum fetuin-A levels and F/A ratio found in obese participants may be considered as new clinical determinants that could be regulated through body composition and nutrition.

  • 
  İncele
• MakaleAçık Erişim2024/r/gumushane/12758
  Chemical Content by LC–MS/MS, Antiglaucoma, and Antioxidant Activity of Propolis Samples From Different Regions of Türkiye

  İzol, Ebubekir; Bursal, Ercan; Yapıcı, İsmail; Abdullah Yilmaz, Mustafa; Yilmaz, İsa; Gülçin, İlhami

  Propolis is a sticky substance produced by bees because of the reaction of beeswax, pollen, and bee enzymes. Particularly, their biological activity and chemical content attract attention. Thus, in this study, the total amount of phenolic and flavonoid substances, Fe3+-Fe2+, Cu2+ (cupric ions reducing activity [CUPRAC]), and Fe3+-TPTZ (ferric ions reducing antioxidant power [FRAP]) reducing, and DPPH• and ABTS•+ scavenging assays in vitro antioxidant properties of propolis samples obtained from four different provinces of Türkiye were determined. In addition, the chemical content of propolis samples was quantitatively determined by LC–MS/MS, and the antiglaucoma property was revealed by hCAII enzyme inhibition. Propolis samples from Ordu presented the highest amounts of total phenolic and flavonoid content (492.3 ± 5.8 and 96.1 ± 2.1, respectively) and also highest antioxidant activity (DPPH• and ABTS•+ IC50 [μg/mL]: 8.884 ± 0.84 and 4.589 ± 0.80, respectively; Fe+3, CUPRAC, and FRAP: 1.051 ± 0.012, 1.021 ± 0.008, and 0.957 ± 0.007 μg/mL, respectively). hCAII enzyme inhibition was highest in Muş propolis (IC50 [μg/mL]: 8.6) as determined. By LC–MS/MS, 53 different components were screened and 35 bioactive components were determined. According to the results, propolis was found to be a raw material because it contains high concentrations of acacetin, chrysin, caffeic acid, and quinic acid (123.824, 24.759, 47.779, and 16.32 mg analyte/g extract, respectively).

  • 
  İncele
• MakaleAçık Erişim2020/r/gumushane/13234
  An assessment of phenolic profiles, fatty acid compositions, and biological activities of two Helichrysum species: H. plicatum and H. chionophilum

  Acet, Tuba; Ozcan, Kadriye; Zengin, Gokhan

  In the present study, we aimed to search and compare the biological activities of the ethanol (EtOH), methanol (MeOH), and ethylacetate (EtOAc) solvent extracts of the flower, stem, and root parts of two Helichrysum plants (H. chionophilum (Hc) and H. plicatum subsp. plicatum (Hp)). The antioxidant properties were determined by using (2,2-diphenyl-1-picrylhydrazyl) (DPPH) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. The enzyme inhibitory effects of the extracts were investigated on butyrylcholinesterase (BChE), acetylcholinesterase (AChE), α-glucosidase, and α-amylase. Palmitic acid (C 16:0) was also determined as major fatty acids in the tested oils (31.21%–67.68%). In both plants, it was found that the EtOAc extracts of the flowers had a strong antioxidant and enzyme inhibitory effect. In conclusion, the results obtained in the present study showed that H. chionophilum and H. plicatum can be seen as a promising source for the natural bioactive compounds that can be used in therapeutic applications. Practical applications: The members of the genus Helichrysum have been widely taken for therapeutic purposes in traditional medicine as well as food. In this context, we investigated the chemical characterization and biological activities of two Helichrysum species extracts (H. chionophilum and H. plicatum subsp. plicatum). Antioxidant capacity, enzyme inhibition and anti-microbial effects were tested for biological activities. Chemical characterization was identified by high performance liquid chromatography (HPLC) (for phenolic) and gas chromatography-flame ioanization detector (GC-FID) (for fatty acids). Based on our findings, the species may be valuable for designing novel food products.

  İncele