Sürdürülebilir Kalkınma Amaçları

• 4

• MakaleAçık Erişim2025Scopus SJRQ2/r/gumushane/12459
  The biological activity and phenolic content of endemic Astragalus baibutensis

  Ozcan, Kadriye; Acet, Tuba; Yiğittürk, Gürkan

  Astragalus baibutensis, is a plant endemic to Turkey, belonging to Fabaceae family, that is used as a folk medicine and food in this country. The purpose of this study is to find out the biological activities such as antioxidant, antimicrobial, enzyme inhibitory and DNA protective activity and also analyse the phenolic contents of the plant. To determine of antioxidant activity ABTS and DPPH assays were performed. Disc diffusion and microdilution methods were used to evaluate the antimicrobial activity. Additionally, enzyme inhibitory activities such as α-amylase, α-glucosidase, and tyrosinase were determined by using spectrophotometric methods. DNA protective activity was detected using pUC19 Plazmid DNA. The antiproliferative effect on SKOV-3 human ovarian cancer cells was examined by WST-8 assay. In addition, the phenolic contents were analysed by using HPLC. The biological activity of ethyl acetate extract of the root was found to be higher than those of other extracts. This extract had the highest amount of apigenin as phenolic compound. On the other hand, extracts showed moderate inhibition against the tested microorganism and antiproliferative properties in a concentration-dependent manner toward ovarian cancer cells. According to the results, Astragalus baibutensis has the potential for pharmaceutical and food additive usage.

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  AtıfScopus1·WoS1·OpenAlex1

  İncele
• MakaleYalnız künye2022Scopus SJRQ2/r/gumushane/14668
  Chemical profile, antioxidant, antimicrobial, and enzyme inhibition activities of Pilosella hoppeana subsp. cilicica

  Acet, Tuba

  Pilosella hoppeana subsp. cilicica has been traditionally used for healing many diseases in folk medicine in Anatolia. However, there are, to date, no studies aimed to verify this local usage with scientific data. In the present study, phenolic content and biological activities such as the antioxidant, antimicrobial and enzyme inhibition activities of different extracts of this plant’s aerial part were investigated for the first time. According to the obtained data, the biological activity of methanol extract was found to be significantly higher than the others. Moreover, the major components detected were chlorogenic acid (61.79 mg g−1 extract), apigenin (2.727 mg g−1 extract), and luteolin (1.110 mg g−1 extract) in this extract. This extract showed a strong enzyme inhibitory activity including α-amylase (96.66 mmol ACAE g−1 extract), α-glucosidase (17.27 mmol ACAE g−1 extract), AChE (10.42 mg GALE g−1 extract), and BChE (102.35 mg GALE g−1 extract). On the other hand, the extract exhibited remarkable antimicrobial effect on Yersinia pseudotuberculosis, and Staphylococcus epidermidis (64 µg/mL MIC value); intermediate (128–256 µg/mL MIC value) antimicrobial activity on Proteus vulgaris, Listeria innocua, and vancomycin-resistant Enterococcus faecium. To sum up, it is clearly seen that Pilosella hoppeana subsp. cilicica has significant potential as a source of bioactive compounds in pharmaceutical industries.

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  AtıfScopus3·WoS3·OpenAlex5

  İncele
• MakaleYalnız künye2021Scopus SJRQ2/r/gumushane/14754
  Determining the phenolic components by using HPLC and biological activity of Centaurea triumfetti

  Acet, Tuba

  Centaurea species belonging to Asteraceae family are traditionally used herbs in treating many diseases in Anatolia. In the present study, I aimed to determine the biological activities and phenolic contents of the extracts of the aerial parts of Centaurea triumfetti collected from areas with extreme climatic conditions. The determination of antioxidant activity was performed using in vitro assays such as ABTS (2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) and DPPH (1,1-diphenyl-2-picrylhydrazyl). Disc diffusion and microdilution methods were used for determine of antimicrobial activity. Besides that, the diabetes-related inhibition activities of enzymes such as alpha-glucosidase and alpha-amylase were determined using colorimetric methods. The phenolic components of the extracts were analysed by using HPLC. The biological activity of ethyl acetate extracts of the stem was found to be higher those than that of other extracts. This extract was also found to have high antimicrobial and enzyme inhibition activity. Moreover, the major component of the extract was found to be chlorogenic acid. In conclusion, these results suggest that C. triumfetti has the potential for pharmaceutical usage.

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  AtıfScopus18·WoS21·OpenAlex17

  İncele
• MakaleYalnız künye2021Scopus SJRQ2/r/gumushane/14700
  Biological activity of Psephellus appendicigerus as a natural agent

  Acet, Tuba; Özcan, Kadriye; Sefalı, Abdurrahman

  Psephellus appendicigerus, is a plant endemic to Turkey, belonging to the Asteraceae family, which is traditionally used in folk medicine in Turkey. The aim of this study is to reveal for the first time the biological activities of this plant and evaluate its potential use as a natural product. For this reason, different extracts (ethanol, methanol, ethyl acetate, and hexane) of the plant’s aerial parts were used in determining the antioxidant (2,20-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid and 1,1-diphenyl-2-picrylhydrazyl assays), antimicrobial (disc diffusion and microdilution methods), and enzyme inhibition activity (calorimetric methods) related to diabetes (α-amylase and α-glucosidase). Moreover, the phenolic constituents were analysed by high performance liquid chromatography. Methanol extract of the plant exhibited significant biological activity compared to other extracts. This extract had the highest amount of chlorogenic acid as phenolic compound. In addition, all extracts showed inhibition against at least one test microorganism. According to the results, P. appendicigerus has the potential to be used as a natural antioxidant, antimicrobial and enzyme inhibitor.

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  AtıfScopus2·WoS2·OpenAlex3

  İncele