Sürdürülebilir Kalkınma Amaçları

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• MakaleAçık Erişim2026/r/gumushane/13879
  Investigation of the Antioxidant, Antibacterial, and Food Safety Potential of Silver and Copper Nanoparticles Green Synthesized Using Pointed Cherry Laurel (Prunus laurocerasus L.) Extract

  Kobya, Havva Nur; Gül, Abdulkadir; Karpuz, Ömer; Baltacı, Cemalettin

  This study reports the green synthesis of silver (PFAgNPs) and copper (PFCuNPs) nanoparticles using an aqueous extract of pointed cherry laurel (Prunus laurocerasus L.) fruit (PF-E) and explores their applicability in food safety. The NPs were characterized via UV–Vis spectroscopy, FT-IR, XRD, SEM, EDX, and TEM confirming their successful formation and morphology. Total phenolic content (TPC), flavonoid content (TFC), and antioxidant capacity (TAC) were measured for both PF-E and the synthesized NPs. While PFCuNPs exhibited higher TPC, PFAgNPs showed superior TFC and TAC. Notably, antioxidant assays (DPPH, ABTS, FRAP) indicated stronger radical scavenging activity for PFCuNPs. Antibacterial tests against Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, and Pseudomonas aeruginosa revealed broad-spectrum activity of PFAgNPs, with the highest inhibition against E. coli (19.4 ± 0.3 mm), whereas PFCuNPs showed dose-dependent efficacy, strongest against E. faecalis (9.6 ± 0.2 mm). LC–MS/MS analysis of the PF-E extract revealed quinic acid (63.42 mg/g), chlorogenic acid (2.38 mg/g), protocatechuic acid (0.07 mg/g), protocatechuic aldehyde (0.04 mg/g), hesperidin (0.03 mg/g), and naringenin (0.02 mg/g) as major components. The adsorption efficiencies of the synthesized PFAgNPs and PFCuNPs were evaluated against the toxic food contaminants patulin and HMF using both standard aqueous solutions and real food systems (apple juice). In standard aqueous solutions, PFAgNPs achieved removal efficiencies of 70.96% for patulin and 52.22% for HMF, while PFCuNPs showed 61.08% and 36.38%, respectively, at 5 g/L after 240 min. In real food systems, the adsorption efficiencies decreased, with PFAgNPs and PFCuNPs removing 34.24% and 32.90% of patulin, and 39.09% and 31.87% of HMF, respectively. The adsorption kinetics of HMF and patulin onto PFCuNP and PFAgNP were found to conform to both the pseudo-first-order and pseudo-second-order kinetic models for aqueous solutions as well as real samples. These findings confirm that the biosynthesized NPs offer dual functionality as antibacterial agents and toxin adsorbents, presenting a green, sustainable approach to food safety enhancement.

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• MakaleAçık Erişim2026/r/gumushane/13602
  Modulation of Hot Pepper Pungency by Melt Dispersion Encapsulation: Role of Wax Type on Microcapsule Structure, Bioactive Compound Content, and Antioxidant Activity

  İncegül, Yasemin; Berktaş, Serap; Çam, Mustafa

  In this study, it was aimed to suppress pungency and obtain a new product by using carnauba, beeswax, and their mixture in the encapsulation of hot pepper oleoresin. Melt dispersion technique was used in production and physical, chemical, and structural analyzes of the obtained capsules were examined. The suppression of the pungency sensation was determined by sensory analysis, and it was determined that the beeswax-based capsule (BW50) was the most effective formulation in suppressing pungency perception (0.83). When looking at the sensory and Scoville Heat Units (SHU) results, there is a parallelism, and it can be stated that the pungency sensation is suppressed. The highest encapsulation efficiency values were observed in CW20 (91.92%), BW20 (90.69%), BC20 (86.92%), and CW30 (86.49%) capsules. When capsule bioactivities were examined, beeswax capsules showed the highest values, while carnauba wax capsules generally showed the lowest values. As expected, as the amount of oleoresin in the capsules increased, the capsules exhibited higher phenolic, flavonoid, carotenoid, and antioxidant activity scores. SEM, FT-IR, and TGA determined that the encapsulation process did not cause any adverse structural reactions in oleoresin. This study broadened the potential application of encapsulated hot pepper oleoresin in food formulations, particularly in functional foods and products intended for consumers sensitive to pungency, by converting it into a more tolerable form.

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• MakaleYalnız künye2026/r/gumushane/13542
  Green Synthesis of Multifunctional Ag/ZnO Nanoparticles Using Vitis labrusca L. Extract: Bioactivity, Malachite Green Adsorption, and HMF Removal

  Hammoudeh, Rama; Bozbeyoğlu, Pınar; Karpuz, Ömer; Gül, Abdulkadir; Ejder, Nebahat; Baltacı, Cemalettin

  In this study, Ag/ZnO nanoparticles (Ag/ZnO NPs) were synthesized via a green route using Vitis labrusca L. extract and evaluated for their bioactive, environmental performance. BM-1 and BM-2 were prepared under different precursor/extract ratios and pH conditions to assess the influence of synthesis parameters. UV–Vis, FT-IR, XRD, HRTEM, DSC, and EDX analyses indicated the successful formation of Ag/ZnO nanostructures with distinct physicochemical features. HRTEM analysis determined that BM-1 exhibited an average particle diameter of 23.18 ± 11.84 nm, while BM-2 demonstrated a mean size of 6.14 ± 1.30 nm. The corresponding relative size-dispersion values were 0.261 for BM-1 and 0.045 for BM-2, indicating a more uniform particle size distribution for BM-2. Antioxidant assays showed that BM-2 exhibited the strongest overall antioxidant performance in DPPH, ABTS•⁺, Fe3+, and Cu2+ assays. Both NPs exhibited antibacterial activity against Gram-positive and Gram-negative bacteria and showed stronger effects than the grape extract alone. Cytotoxicity assays revealed dose-dependent inhibition in A549 and HeLa cancer cells, while lower toxicity was observed in normal ARPE-19 cells, suggesting cell-type-dependent biological response. In adsorption experiments, the NPs effectively removed malachite green, with isotherm analysis providing stronger support for the Langmuir model for BM-1 and comparable Langmuir and Freundlich fits for BM-2, while the pseudo-second-order model provided the best description of the adsorption kinetics. The maximum dye adsorption capacities were 85.5 mg/g for BM-1 and 101.3 mg/g for BM-2. Under optimum conditions (5.0 g/L NPs, 240 min), the highest HMF removal efficiencies reached 44.49% for BM-2 in standard aqueous solutions, whereas competitive matrix interactions in apple juice limited the maximum efficiency to 29.27%. Overall, this study highlights the value of integrating green synthesis, bioactivity, dye adsorption, and food-related contaminant removal within a single Ag/ZnO nanoplatform.

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• MakaleYalnız künye2026/r/gumushane/12297
  Beeswax Waste-Derived Pyrolysis Liquid as a Novel Multifunctional Bioactive Fraction: An Integrated Chemical and Biological Evaluation

  Gül, Abdulkadir

  In this study, beeswax waste, a low-value by-product of apiculture, was converted into a liquid fraction with bioactive potential through pyrolysis-assisted thermochemical transformation. The LC–MS/MS-based chemical profile, total phenolic content (TPC), total flavonoid content (TFC), antioxidant responses, and antibacterial, antidiabetic, anti-inflammatory, and anticholinesterase activities of beeswax waste pyrolysis liquid (BWPL) were investigated. The TPC and TFC values were determined as 95.30 ± 1.13 mg GAE/L and 51.67 ± 1.12 mg QE/L, respectively. LC–MS/MS analysis revealed benzoic acid and vanillin as the predominant compounds, along with various phenolic acids and flavonoid derivatives. BWPL showed 51.83 ± 1.51% inhibition in the ABTS•⁺ assay and 9.71 ± 0.28% inhibition in the DPPH assay. It also exhibited measurable redox capacity in Fe3+ and Cu2+ reducing power assays. In antibacterial assays, BWPL produced inhibition zones against all tested strains at 1 mg/mL and against E. coli and E. faecalis at 500 µg/mL. The IC50 values of BWPL for 5-LOX and α-glucosidase were 38.17 µg/mL and 1503.0 µg/mL, respectively. In addition, the IC50 values for AChE and BChE were calculated as 112.38 ± 0.87 mg/mL and 13.01 ± 0.42 mg/mL, respectively. PCA and correlation analyses indicated the multidimensional relationships between the LC–MS/MS-based chemical profile and biological activities. Overall, the pyrolytic conversion of beeswax waste into a chemically complex liquid fraction provides a preliminary scientific basis for waste valorization and for further investigation of pyrolysis-derived bioactive fractions.

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• MakaleYalnız künye2014/r/gumushane/15413
  Response Surface Methodology Study for Optimization of Effects of Fiber Level, Frying Temperature, and Frying Time on Some Physicochemical, Textural, and Sensory Properties of Wheat Chips Enriched with Apple Fiber

  Kayacier, Ahmed; Yüksel, Ferhat; Karaman, Safa

  In this study, wheat chips enriched with apple fiber were produced, and response surface methodology was used for the determination of the simultaneous effects of processing variables selected as fiber level (0-15 %), frying temperature (160-180 °C), and frying time (40-60 s) on some physicochemical, textural, and sensorial properties of chips. Ridge analysis was conducted to determine the optimum levels of processing variables. Predictive regression equations with high coefficient of determination (R 2 ≥ 0.728) were constructed. Addition of apple fiber increased the dry matter; ash content; L, a, and b values of samples, while increase of frying temperature caused decrease of the hardness values. Overall acceptability of chips enriched with apple fiber decreased with the increase of frying temperature, but wheat chips enriched with apple fiber and fried at low temperatures received highest sensory score. Ridge analysis showed that maximum taste score would be attained at fiber level = 15 %, frying temperature = 170 °C and frying time = 40 s.

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